Score: 7.00 |
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| Title: Brittle Culm 12 , a dual-targeting kinesin-4 protein , controls cell-cycle progression and wall properties in rice .
| | Author: Zhang M Zhang B Qian Q Yu Y Li R Zhang J Liu X Zeng D Li J Zhou Y | | Journal: Plant J Citation: V : 63 P : 312-28 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20444225 Accession (PMID): 20444225 | | Abstract: Kinesins are encoded by a large gene family involved in many basic processes of plant development .
However , the number of functionally identified kinesins in rice is very limited .
Here , we report the functional characterization of Brittle Culm12 ( BC12 ) , a gene encoding a kinesin-4 protein . bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition .
BC12 is expressed mainly in it issues undergoing cell division and secondary wall thickening .
In vitro biochemical analyses verified BC12 as an authentic motor protein .
This protein was present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division .
Mitotic microtubule array comparison , flow cytometric analysis and expression assays of cyclin-dependent kinase ( CDK ) complexes in root-tip cells showed that cell-cycle progression is affected in bc12 mutants .
BC12 is very probably regulated by CDKA ; 3 based on yeast two-hybrid and microarray data .
Therefore , BC12 functions as a dual-targeting kinesin protein and is implicated in cell-cycle progression , cellulose microfibril deposition and wall composition in the monocot plant rice .
| Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Here , we report the functional characterization of Brittle Culm12 ( BC12 ) , a gene encoding a kinesin-4 protein . bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition .
[ Sen. 4, subscore: 1.00 ]: BC12 is expressed mainly in it issues undergoing cell division and secondary wall thickening .
[ Sen. 5, subscore: 1.00 ]: In vitro biochemical analyses verified BC12 as an authentic motor protein .
[ Sen. 7, subscore: 1.00 ]: Mitotic microtubule array comparison , flow cytometric analysis and expression assays of cyclin-dependent kinase ( CDK ) complexes in root-tip cells showed that cell-cycle progression is affected in bc12 mutants .
[ Sen. 8, subscore: 1.00 ]: BC12 is very probably regulated by CDKA ; 3 based on yeast two-hybrid and microarray data .
[ Sen. 9, subscore: 1.00 ]: Therefore , BC12 functions as a dual-targeting kinesin protein and is implicated in cell-cycle progression , cellulose microfibril deposition and wall composition in the monocot plant rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Mutation of rice BC12/GDD1 , which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter , leads to dwarfism with impaired cell elongation .
| | Author: Li J Jiang J Qian Q Xu Y Zhang C Xiao J Du C Luo W Zou G Chen M Huang Y Feng Y Cheng Z Yuan M Chong K | | Journal: Plant Cell Citation: V : 23 P : 628-40 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21325138 Accession (PMID): 21325138 | | Abstract: The kinesins are a family of microtubule-based motor proteins that move directionally along microtubules and are involved in many crucial cellular processes , including cell elongation in plants .
Less is known about kinesins directly regulating gene transcription to affect cellular physiological processes .
Here , we describe a rice ( Oryza sativa ) mutant , gibberellin-deficient dwarf1 ( gdd1 ) , that has a phenotype of greatly reduced length of root , stems , spikes , and seeds .
This reduced length is due to decreased cell elongation and can be rescued by exogenous gibberellic acid ( GA ) treatment .
GDD1 was cloned by a map-based approach , was expressed constitutively , and was found to encode the kinesin-like protein BRITTLE CULM12 ( BC12 ) .
Microtubule cosedimentation assays revealed that BC12/GDD1 bound to microtubules in an ATP-dependent manner .
Whole-genome microarray analysis revealed the expression of ent-kaurene oxidase ( KO2 ) , which encodes an enzyme involved in GA biosynthesis , was downregulated in gdd1 .
Electrophoretic mobility shift and chromatin immunoprecipitation assays revealed that GDD1 bound to the element ACCAACTTGAA in the KO2 promoter .
In addition , GDD1 was shown to have transactivation activity .
The level of endogenous GAs was reduced in gdd1 , and the reorganization of cortical microtubules was altered .
Therefore , BC12/GDD1 , a kinesin-like protein with transcription regulation activity , mediates cell elongation by regulating the GA biosynthesis pathway in rice .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: GDD1 was cloned by a map-based approach , was expressed constitutively , and was found to encode the kinesin-like protein BRITTLE CULM12 ( BC12 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
