Abstract: Bacillus amyloliquefaciens DS11 phytase ( DS11 phytase ) and Aspergillus ficuum phytase ( AF phytase ) activities were investigated by measuring the release of phosphate from phytate in animal feedstuff such as wheat bran , corn meal , soybean meal and rice flour at pH 5 and 7 .
In all the tested feedstuff , the enzymatic activity of DS11 phytase was more active at pH 7 , but that of AF phytase was more active at pH 5 .
From these results , the phytate in the gastrointestinal tract could be degraded in the small intestine or stomach by DS11 or AF phytase , respectively .
In conclusion , the results presented in this paper indicated that different combination ratios of DS11 and AF phytase , depending on the kind of feedstuff , might effectively induce more enzymatic activity both in the stomach and small intestine in terms of the pH of the gastrointestinal tract . |