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Score: 7.00 |
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| Title: Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses .
| | Author: Peres A Churchman ML Hariharan S Himanen K Verkest A Vandepoele K Magyar Z Hatzfeld Y Van Der Schueren E Beemster GT Frankard V Larkin JC Inze D De Veylder L | | Journal: J Biol Chem Citation: V : 282 P : 25588-96 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17599908 Accession (PMID): 17599908 | | Abstract: The EL2 gene of rice ( Oryza sativa ) , previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function , was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM ) gene of Arabidopsis thaliana .
Iterative two-hybrid screens , in vitro pull-down assays , and fluorescence resonance energy transfer analyses showed that Orysa ; EL2 binds the cyclin-dependent kinase ( CDK ) CDKA1 ; 1 and D-type cyclins .
No interaction was observed with the plant-specific B-type CDKs .
The amino acid motif ELERFL was identified to be essential for cyclin , but not for CDK binding .
Orysa ; EL2 impaired the ability of Orysa ; CYCD5 ; 3 to complement a budding yeast ( Saccharomyces cerevisiae ) triple CLN mutant , whereas recombinant protein inhibited CDK activity in vitro .
Moreover , Orysa ; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis , unequivocally demonstrating that Orysa ; EL2 operates as a cell cycle inhibitor .
Orysa ; EL2 mRNA levels were induced by cold , drought , and propionic acid .
Our data suggest that Orysa ; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Moreover , Orysa ; EL2 was able to rescue the multicellular trichome phenotype of sim mutants of Arabidopsis , unequivocally demonstrating that Orysa ; EL2 operates as a cell cycle inhibitor .
[ Sen. 1, subscore: 1.00 ]: The EL2 gene of rice ( Oryza sativa ) , previously classified as early response gene against the potent biotic elicitor N-acetylchitoheptaose and encoding a short polypeptide with unknown function , was identified as a novel cell cycle regulatory gene related to the recently reported SIAMESE ( SIM ) gene of Arabidopsis thaliana .
[ Sen. 2, subscore: 1.00 ]: Iterative two-hybrid screens , in vitro pull-down assays , and fluorescence resonance energy transfer analyses showed that Orysa ; EL2 binds the cyclin-dependent kinase ( CDK ) CDKA1 ; 1 and D-type cyclins .
[ Sen. 5, subscore: 1.00 ]: Orysa ; EL2 impaired the ability of Orysa ; CYCD5 ; 3 to complement a budding yeast ( Saccharomyces cerevisiae ) triple CLN mutant , whereas recombinant protein inhibited CDK activity in vitro .
[ Sen. 7, subscore: 1.00 ]: Orysa ; EL2 mRNA levels were induced by cold , drought , and propionic acid .
[ Sen. 8, subscore: 1.00 ]: Our data suggest that Orysa ; EL2 encodes a new type of plant CDK inhibitor that links cell cycle progression with biotic and abiotic stress responses .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides : differences in the signal transduction pathways leading to the activation of elicitor-responsive genes .
| | Author: Nishizawa Y Kawakami A Hibi T He DY Shibuya N Minami E | | Journal: Plant Mol . Biol . Citation: V : 39 ( 5 ) P : 907-14 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10344196 Accession (PMID): 10344196 | | Abstract: Expression patterns of chitinase transcripts induced by N-acetylchitooligosaccharide elicitor were analyzed by northern blot hybridization in order to reveal a signal transduction pathway leading to the activation of class I chitinase genes ( Cht-1 and Cht-3 ) , which may play an important role in producing N-acetylchitooligosaccharide elicitor .
The transcription level of both genes was enhanced in response to N-acetylchitooligosaccharides larger than pentaose at subnanomolar concentrations .
These structure and dose dependencies were consistent not only with those for a 75 kDa high-affinity binding protein for N-acetylchitooligosaccharide elicitor in the plasma membrane , but also with other series of cellular responses including phytoalexin production and the expression of elicitor-responsive genes ( EL2 , EL3 ) .
Therefore , the elicitor signal to evoke these cellular responses including the activation of the chitinase genes could be common and transmitted into cells through the 75 kDa protein .
However , the signal transduction pathway for the activation of the chitinase gene appeared to diverge from those for the other elicitor-responsive genes shortly after the signal perception .
It was shown that the induction of chitinase expression by N-acetylchitooligosaccharide would require protein phosphorylation , but not de novo protein synthesis .
The oxidative burst was demonstrated not to be necessary for transcriptional induction of the all four elicitor-responsive genes ( Cht , PAL , EL2 , EL3 ) by N-acetylchitooligosaccharide . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: These structure and dose dependencies were consistent not only with those for a 75 kDa high-affinity binding protein for N-acetylchitooligosaccharide elicitor in the plasma membrane , but also with other series of cellular responses including phytoalexin production and the expression of elicitor-responsive genes ( EL2 , EL3 ) .
[ Sen. 7, subscore: 1.00 ]: The oxidative burst was demonstrated not to be necessary for transcriptional induction of the all four elicitor-responsive genes ( Cht , PAL , EL2 , EL3 ) by N-acetylchitooligosaccharide .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Induction of resistance against rice blast fungus in rice plants treated with a potent elicitor , N-acetylchitooligosaccharide .
| | Author: Tanabe S Okada M Jikumaru Y Yamane H Kaku H Shibuya N Minami E | | Journal: Biosci . Biotechnol . Biochem . Citation: V : 70 ( 7 ) P : 1599-605 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16861793 Accession (PMID): 16861793 | | Abstract: The mode of action of a potent elicitor , N-acetylchitooligosaccharide , in rice plants was examined .
In intact seedlings , no significant uptake of the elicitor via the roots was observed within 3 h , whereas rapid uptake was observed in excised leaves .
Rapid and transient expression of an elicitor-responsive gene , EL2 , was induced in the leaves of intact seedlings sprayed with the elicitor or in the roots and leaves of intact seedlings by immersing roots in the elicitor solution .
Histochemical analysis indicated that EL2 was expressed in cells exposed to the elicitor of root and leaves .
In seedlings treated with the elicitor for 1 d or longer , hyphal growth of rice blast fungus was significantly delayed , and an accumulation of auto-fluorescence around the infection site was observed .
Two defense-related genes , PR-1 and PR-10 ( PBZ1 ) , were induced in a systemic and local manner by elicitor treatment , in correlation with the induction of resistance against rice blast fungus .
N-Acetylchitoheptaose did not inhibit the hyphal growth of the fungi .
These results indicate the occurrence of systemic signal transmission from N-acetylchitooligosaccharide in rice plants . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Rapid and transient expression of an elicitor-responsive gene , EL2 , was induced in the leaves of intact seedlings sprayed with the elicitor or in the roots and leaves of intact seedlings by immersing roots in the elicitor solution .
[ Sen. 4, subscore: 1.00 ]: Histochemical analysis indicated that EL2 was expressed in cells exposed to the elicitor of root and leaves .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Two novel genes rapidly and transiently activated in suspension-cultured rice cells by treatment with N-acetylchitoheptaose , a biotic elicitor for phytoalexin production .
| | Author: Minami E Kuchitsu K He DY Kouchi H Midoh N Ohtsuki Y Shibuya N | | Journal: Plant Cell Physiol .
Citation: V : 37 ( 4 ) P : 563-7 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8759920 Accession (PMID): 8759920 | | Abstract: By using subtracted probes , two cDNA clones of rice , EL2 and EL3 , were isolated as genes responsive within 6 min to N-acetylchitoheptaose , a potent biotic elicitor for phytoalexin biosynthesis .
Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: By using subtracted probes , two cDNA clones of rice , EL2 and EL3 , were isolated as genes responsive within 6 min to N-acetylchitoheptaose , a potent biotic elicitor for phytoalexin biosynthesis .
[ Sen. 2, subscore: 1.00 ]: Analyses of the sequence of the cDNAs showed that both of EL2 and EL3 encoded basic proteins with no significant similarities to those of known genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells .
| | Author: Che FS Nakajima Y Tanaka N Iwano M Yoshida T Takayama S Kadota I Isogai A | | Journal: J Biol . Chem . Citation: V : 275 ( 41 ) P : 32347-56 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10922369 Accession (PMID): 10922369 | | Abstract: The host range of Pseudomonas avenae is wide among monocotyledonous plants , but individual strains can infect only one or a few host species .
The resistance response of rice cells to pathogens has been previously shown to be induced by a rice-incompatible strain , N1141 , but not by a rice-compatible strain , H8301 .
To clarify the molecular mechanism of the host specificity in P avenae , a strain-specific antibody that was raised against N1141 cells and then absorbed with H8301 cells was prepared .
When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody , only a flagellin protein was detected .
Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment , whereas the H8301 flagellin did not .
The hypersensitive cell death could be blocked by pretreatment with anti-N1141 flagellin antibody .
Furthermore , a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene , which is thought to be one of the defense-related genes .
These results demonstrated that the resistance response in cultured rice cells is induced by the flagellin existing in the incompatible strain of P avenae but not in the flagellin of the compatible strain . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Furthermore , a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene , which is thought to be one of the defense-related genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
