|
Score: 12.00 |
|---|
| Title: Cloning and characterization of barley caryopsis FCA .
| | Author: Kumar S Jiang S Jami SK Hill RD | | Journal: Physiol Plant Citation: V : 143 P : 93-106 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21645000 Accession (PMID): 21645000 | | Abstract: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis .
FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) .
We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv .
McLeod ) .
The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain .
Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog .
In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP .
FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed .
ABA inhibition of germination inhibited the decline of barley embryo FCA .
Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity .
Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog .
[ Sen. 10, subscore: 2.00 ]: Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity .
[ Sen. 11, subscore: 2.00 ]: Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation .
[ Sen. 1, subscore: 1.00 ]: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis .
[ Sen. 2, subscore: 1.00 ]: FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) .
[ Sen. 3, subscore: 1.00 ]: We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv .
[ Sen. 7, subscore: 1.00 ]: In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP .
[ Sen. 8, subscore: 1.00 ]: FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed .
[ Sen. 9, subscore: 1.00 ]: ABA inhibition of germination inhibited the decline of barley embryo FCA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
|---|
| Title: Conservation and divergence of FCA function between Arabidopsis and rice .
| | Author: Lee JH Cho YS Yoon HS Suh MC Moon J Lee I Weigel D Yun CH Kim JK .
| | Journal: Plant Mol . Biol . Citation: V : 58 ( 6 ) P : 823-38 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16240176 Accession (PMID): 16240176 | | Abstract: Although several genes have been identified in rice which are functionally equivalent to the flowering time genes in Arabidopsis , primarily genes involved in the photoperiod pathway , little data is available regarding the genes that function in the autonomous pathway in rice .
In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA .
The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA .
However , the genetic structure of OsFCA is , in general , similar to that of FCA .
RT-PCR and in silico analyses also showed that alternative splicing and polyadenylation at intron3 were conserved in the genetic expression of OsFCA .
We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene .
The beta and gamma transcripts of the OsFCA gene were detected via Northern analysis in the leaves , roots , and flowers of the plant .
Flowers in younger stages exhibited higher transcript levels .
These data suggest that intron3 may constitute a primary control point in the OsFCA pre-mRNA processing of rice .
The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome .
The constitutive expression of OsFCA resulted in no downregulation of FLC , but did result in the weak upregulation of SOC1 in the transgenic Arabidopsis .
OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA .
All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis . | Matching Sentences:
[ Sen. 10, subscore: 2.00 ]: The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome .
[ Sen. 12, subscore: 2.00 ]: OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA .
[ Sen. 2, subscore: 1.00 ]: In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA .
[ Sen. 3, subscore: 1.00 ]: The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA .
[ Sen. 4, subscore: 1.00 ]: However , the genetic structure of OsFCA is , in general , similar to that of FCA .
[ Sen. 13, subscore: 1.00 ]: All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: OsFY , a homolog of AtFY , encodes a protein that can interact with OsFCA-gamma in rice ( Oryza sativa L ) .
| | Author: Lu Q Xu ZK Song RT .
| | Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 38 ( 7 ) P : 492-9 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16820865 Accession (PMID): 16820865 | | Abstract: FCA and FY are flowering time related genes involved in the autonomous flowering pathway in Arabidopsis .
FCA interacts with FY to regulate the alternative processing of FCA pre-mRNA .
The FCA/FY interaction is also required for the regulation of FLC expression , a major floral repressor in Arabidopsis .
However , it is not clear if the regulation of this autonomous flowering pathway is also present in monocot plants , such as rice .
Recently , alternative RNA processing of OsFCA was observed in rice , which strongly suggested the existence of an autonomous flowering pathway in rice .
In this work , we cloned the cDNA of the autonomous flowering pathway gene OsFY from rice .
The predicted OsFY protein contained a conserved 7 WD-repeat region and at least two Pro-Pro-Leu-Pro motifs compared to Arabidopsis FY .
The protein-protein interaction between OsFY and OsFCA-gamma , the key feature of their gene function , was also demonstrated using the yeast two-hybrid system .
The GenBank database search provided evidence of expression for other autonomous pathway gene homologs in rice .
These results indicate that the autonomous flowering pathway is present in monocots , and the regulation through FY and FCA interaction is conserved between monocots and dicots . | Matching Sentences:
[ Sen. 2, subscore: 2.00 ]: FCA interacts with FY to regulate the alternative processing of FCA pre-mRNA .
[ Sen. 1, subscore: 1.00 ]: FCA and FY are flowering time related genes involved in the autonomous flowering pathway in Arabidopsis .
[ Sen. 10, subscore: 1.00 ]: These results indicate that the autonomous flowering pathway is present in monocots , and the regulation through FY and FCA interaction is conserved between monocots and dicots .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Overexpression of the rFCA RNA recognition motif affects morphologies modifications in rice ( Oryza sativa L ) .
| | Author: Hong F Attia K Wei C Li K He G Su W Zhang Q Qian X Yang J | | Journal: Biosci Rep Citation: V : 27 P : 225-34 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17597396 Accession (PMID): 17597396 | | Abstract: RNA recognition motifs as important regulators of gene expression are highly conserved in animals and plants .
The FCA floral promotion gene in Arabidopsis encodes a protein , containing two RNA recognition motifs ( RRM ) and a WW protein interaction domain .
Here we isolated FCA cDNA from rice .
FCA in rice ( rFCA ) was homologous to FCA-gamma of Arabidopsis and contained conserved domains .
To investigate the function of RRM domain , fragment RRM1 and RRM2 of rFCA were introduced into rice subspecies Oryza sativa L subsp .
Indica var .
9311 and another rice subspecies Oryza sativa L subsp .
Japonica var . zhonghua11 transformation .
Two transgenic lines exhibited similar phenotypes , flowering time delay , seed size and cell volume of transgenic plants was increased .
These results showed that constitutive expression of RRMs could regulate cellular size .
The patterns of overexpression of two RRM domains and their similar morphologies indicate they may play a same role .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: FCA in rice ( rFCA ) was homologous to FCA-gamma of Arabidopsis and contained conserved domains .
[ Sen. 2, subscore: 1.00 ]: The FCA floral promotion gene in Arabidopsis encodes a protein , containing two RNA recognition motifs ( RRM ) and a WW protein interaction domain .
[ Sen. 3, subscore: 1.00 ]: Here we isolated FCA cDNA from rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: Abscisic acid does not disrupt either the Arabidopsis FCA-FY interaction or its rice counterpart in vitro .
| | Author: Jang YH Lee JH Kim JK | | Journal: Plant Cell Physiol Citation: V : 49 P : 1898-901 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18854333 Accession (PMID): 18854333 | | Abstract: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY .
From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment .
This result prompted us to examine the effect of ABA on the FCA-FY interaction .
In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY .
Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY .
[ Sen. 3, subscore: 1.00 ]: This result prompted us to examine the effect of ABA on the FCA-FY interaction .
[ Sen. 4, subscore: 1.00 ]: In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY .
[ Sen. 5, subscore: 1.00 ]: Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Alternative splicing and expression analysis of OsFCA ( FCA in Oryza sativa L ) , a gene homologous to FCA in Arabidopsis .
| | Author: Du X Qian X Wang D Yang J | | Journal: DNA Seq .
Citation: V : 17 ( 1 ) P : 31-40 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16753815 Accession (PMID): 16753815 | | Abstract: A subtracted cDNAs library was constructed using rice ( Oryza sativa L ) calli cDNA as driver and differentiating calli cDNA as tester .
A novel gene homologous with FCA in Arabidopsis was cloned from rice by screening the SSH ( suppression subtractive hybridization ) library followed by RACE .
Four alternative transcripts of OsFCA were cloned from the leaves of rice , and designated as OsFCA-1 , OsFCA-2 , OsFCA-3 and OsFCA-4 respectively .
OsFCA-1 was homologous to FCA-gamma of Arabidopsis and contained several conserved domains ( two RNA Recognition Motifs and one WW-domain ) .
OsFCA-2 was 102 bp shorter than OsFCA-1 which caused the WW-domain deletion .
The proteins encoded by OsFCA-3 and OsFCA-4 were 101 amino acids shorter than OsFCA-1 at the N-terminal which is a glycine-rich region .
The fluorescence quantitative PCR analysis showed that the mRNA of OsFCA-1 is the most abundant in the four splicing variants of rice FCA , and its expression level is much higher in differentiating calli than in calli .
The expression of OsFCA-1 is steady in the leaves of three different stage , but up-regulated in young spikelet of primary branch-differentiating stage and down-regulated in young spikelet of pistil and stamen-differentiating stage . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: A novel gene homologous with FCA in Arabidopsis was cloned from rice by screening the SSH ( suppression subtractive hybridization ) library followed by RACE .
[ Sen. 4, subscore: 1.00 ]: OsFCA-1 was homologous to FCA-gamma of Arabidopsis and contained several conserved domains ( two RNA Recognition Motifs and one WW-domain ) .
[ Sen. 7, subscore: 1.00 ]: The fluorescence quantitative PCR analysis showed that the mRNA of OsFCA-1 is the most abundant in the four splicing variants of rice FCA , and its expression level is much higher in differentiating calli than in calli .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Survey of rice proteins interacting with OsFCA and OsFY proteins which are homologous to the Arabidopsis flowering time proteins , FCA and FY .
| | Author: Jang YH Park HY Kim SK Lee JH Suh MC Chung YS Paek KH Kim JK | | Journal: Plant Cell Physiol Citation: V : 50 P : 1479-92 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19561057 Accession (PMID): 19561057 | | Abstract: The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis .
It contains two RNA-binding domains and a WW domain .
The FCA protein interacts with FY , a polyadenylation factor , via its WW domain .
We previously characterized a rice gene , OsFCA , which was homologous to FCA .
Here , we found that the OsFCA protein could interact through its WW domain with the following proteins : OsFY , a protein containing a CID domain present in RNA-processing factors such as Pcf11 and Nrd1 ; a protein similar to splicing factor SF1 ; a protein similar to FUSE splicing factor ; and OsMADS8 .
The FY protein is associated with the 3 end processing machinery in Arabidopsis .
Thus , we examined interactions between OsFY and the rice homologs ( OsCstF-50 , -64 and -77 ) of the AtCstF-50 , -64 and -77 proteins .
We found that OsFY could bind OsCstF50 , whereas the OsCstF77 protein could bridge the interaction between OsCstF50 and OsCstF64 .
Taken together , our data suggest that OsFCA could interact with several proteins other than OsFY through its WW domain and may play several roles in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis .
[ Sen. 3, subscore: 1.00 ]: The FCA protein interacts with FY , a polyadenylation factor , via its WW domain .
[ Sen. 4, subscore: 1.00 ]: We previously characterized a rice gene , OsFCA , which was homologous to FCA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Alternative splicing and expression analysis of OsFCA ( FCA in Oryza sativa L ) , a gene homologous to FCA in Arabidopsis .
| | Author: Du X Qian X Wang D Yang J | | Journal: DNA Seq .
Citation: V : 17 ( 1 ) P : 31-40 Year: 2006 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16753815 Accession (PMID): 16753815 | | Abstract: A subtracted cDNAs library was constructed using rice ( Oryza sativa L ) calli cDNA as driver and differentiating calli cDNA as tester .
A novel gene homologous with FCA in Arabidopsis was cloned from rice by screening the SSH ( suppression subtractive hybridization ) library followed by RACE .
Four alternative transcripts of OsFCA were cloned from the leaves of rice , and designated as OsFCA-1 , OsFCA-2 , OsFCA-3 and OsFCA-4 respectively .
OsFCA-1 was homologous to FCA-gamma of Arabidopsis and contained several conserved domains ( two RNA Recognition Motifs and one WW-domain ) .
OsFCA-2 was 102 bp shorter than OsFCA-1 which caused the WW-domain deletion .
The proteins encoded by OsFCA-3 and OsFCA-4 were 101 amino acids shorter than OsFCA-1 at the N-terminal which is a glycine-rich region .
The fluorescence quantitative PCR analysis showed that the mRNA of OsFCA-1 is the most abundant in the four splicing variants of rice FCA , and its expression level is much higher in differentiating calli than in calli .
The expression of OsFCA-1 is steady in the leaves of three different stage , but up-regulated in young spikelet of primary branch-differentiating stage and down-regulated in young spikelet of pistil and stamen-differentiating stage . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Alternative splicing and expression analysis of OsFCA ( FCA in Oryza sativa L ) , a gene homologous to FCA in Arabidopsis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: [ Food crops in Sahelian countries : assessment and challenges ] | | Author: Egg J Wade I | | Journal: Sante Citation: V : 16 P : 271-8 Year: 2006 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17446162 Accession (PMID): 17446162 | | Abstract: Twenty-five years after the beggining of the policies of adjustment and liberalization which is the situation of the food crops in the countries of the Sahel?
The balance-sheet is approached by contrasting the cereals sub sector , facing to a very strong control by the State , and the onion whose growth is related to the increase in the demand of the great urban centers .
The increase in the production of cereals was accompanied by deep changes in the structures .
The market became more efficient and better integrated on a regional scale .
But , the level and the instability of the prices of cereals increased , making difficult the provisioning of the poor .
Clear differences in trajectories appear .
Mali , which implemented a policy of reforming cereals market and successful the revival of rice sector , must manage at the same time the improvement of the competitiveness of its cereals and the access to cereals of the low incomes consumers .
Niger must face the increase in the vulnerability of a great part of the rural households .
The production of onion experienced a spectacular increase in Senegal after the devaluation of franc FCA in response to the urban market .
That of Niger intended mainly for the close countries progressed more slowly .
But in both cases , the regulation of the European onion imports constitutes a paramount stake .
| Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: The production of onion experienced a spectacular increase in Senegal after the devaluation of franc FCA in response to the urban market .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Conservation and divergence of FCA function between Arabidopsis and rice .
| | Author: Lee JH Cho YS Yoon HS Suh MC Moon J Lee I Weigel D Yun CH Kim JK .
| | Journal: Plant Mol . Biol . Citation: V : 58 ( 6 ) P : 823-38 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16240176 Accession (PMID): 16240176 | | Abstract: Although several genes have been identified in rice which are functionally equivalent to the flowering time genes in Arabidopsis , primarily genes involved in the photoperiod pathway , little data is available regarding the genes that function in the autonomous pathway in rice .
In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA .
The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA .
However , the genetic structure of OsFCA is , in general , similar to that of FCA .
RT-PCR and in silico analyses also showed that alternative splicing and polyadenylation at intron3 were conserved in the genetic expression of OsFCA .
We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene .
The beta and gamma transcripts of the OsFCA gene were detected via Northern analysis in the leaves , roots , and flowers of the plant .
Flowers in younger stages exhibited higher transcript levels .
These data suggest that intron3 may constitute a primary control point in the OsFCA pre-mRNA processing of rice .
The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome .
The constitutive expression of OsFCA resulted in no downregulation of FLC , but did result in the weak upregulation of SOC1 in the transgenic Arabidopsis .
OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA .
All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Conservation and divergence of FCA function between Arabidopsis and rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Abscisic acid does not disrupt either the Arabidopsis FCA-FY interaction or its rice counterpart in vitro .
| | Author: Jang YH Lee JH Kim JK | | Journal: Plant Cell Physiol Citation: V : 49 P : 1898-901 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18854333 Accession (PMID): 18854333 | | Abstract: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY .
From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment .
This result prompted us to examine the effect of ABA on the FCA-FY interaction .
In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY .
Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Abscisic acid does not disrupt either the Arabidopsis FCA-FY interaction or its rice counterpart in vitro .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Survey of rice proteins interacting with OsFCA and OsFY proteins which are homologous to the Arabidopsis flowering time proteins , FCA and FY .
| | Author: Jang YH Park HY Kim SK Lee JH Suh MC Chung YS Paek KH Kim JK | | Journal: Plant Cell Physiol Citation: V : 50 P : 1479-92 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19561057 Accession (PMID): 19561057 | | Abstract: The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis .
It contains two RNA-binding domains and a WW domain .
The FCA protein interacts with FY , a polyadenylation factor , via its WW domain .
We previously characterized a rice gene , OsFCA , which was homologous to FCA .
Here , we found that the OsFCA protein could interact through its WW domain with the following proteins : OsFY , a protein containing a CID domain present in RNA-processing factors such as Pcf11 and Nrd1 ; a protein similar to splicing factor SF1 ; a protein similar to FUSE splicing factor ; and OsMADS8 .
The FY protein is associated with the 3 end processing machinery in Arabidopsis .
Thus , we examined interactions between OsFY and the rice homologs ( OsCstF-50 , -64 and -77 ) of the AtCstF-50 , -64 and -77 proteins .
We found that OsFY could bind OsCstF50 , whereas the OsCstF77 protein could bridge the interaction between OsCstF50 and OsCstF64 .
Taken together , our data suggest that OsFCA could interact with several proteins other than OsFY through its WW domain and may play several roles in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Survey of rice proteins interacting with OsFCA and OsFY proteins which are homologous to the Arabidopsis flowering time proteins , FCA and FY .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Cloning and characterization of barley caryopsis FCA .
| | Author: Kumar S Jiang S Jami SK Hill RD | | Journal: Physiol Plant Citation: V : 143 P : 93-106 Year: 2011 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub21645000 Accession (PMID): 21645000 | | Abstract: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis .
FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) .
We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv .
McLeod ) .
The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain .
Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog .
In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP .
FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed .
ABA inhibition of germination inhibited the decline of barley embryo FCA .
Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity .
Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Cloning and characterization of barley caryopsis FCA .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
