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Score: 3.00 |
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| Title: OsIPD3 , an ortholog of the Medicago truncatula DMI3 interacting protein IPD3 , is required for mycorrhizal symbiosis in rice .
| | Author: Chen C Ane JM Zhu H | | Journal: New Phytol Citation: V : 180 P : 311-5 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18761634 Accession (PMID): 18761634 | | Abstract: Medicago truncatula IPD3 ( MtIPD3 ) is an interacting protein of DMI3 ( does not make infections 3 ) , a Ca ( 2+ ) /calmodulin-dependent protein kinase ( CCaMK ) essential for both arbuscular mycorrhizal ( AM ) and rhizobial symbioses .
However , the function of MtIPD3 in root symbioses has not been demonstrated in M truncatula , because of a lack of knockout mutants for functional analysis .
In this study , the availability of IPD3 knockout mutants in rice ( Oryza sativa ) was exploited to test the function of OsIPD3 in AM symbiosis .
Three independent retrotransposon Tos17 insertion lines of OsIPD3 were selected and the phenotypes characterized upon inoculation with the AM fungus Glomus intraradices .
Phenotypic and genetic analyses revealed that the Osipd3 mutants were unable to establish a symbiotic association with G intraradices .
In conclusion , IPD3 represents a novel gene required for root symbiosis with AM fungi in plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Medicago truncatula IPD3 ( MtIPD3 ) is an interacting protein of DMI3 ( does not make infections 3 ) , a Ca ( 2+ ) /calmodulin-dependent protein kinase ( CCaMK ) essential for both arbuscular mycorrhizal ( AM ) and rhizobial symbioses .
[ Sen. 3, subscore: 1.00 ]: In this study , the availability of IPD3 knockout mutants in rice ( Oryza sativa ) was exploited to test the function of OsIPD3 in AM symbiosis .
[ Sen. 6, subscore: 1.00 ]: In conclusion , IPD3 represents a novel gene required for root symbiosis with AM fungi in plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Presence of three mycorrhizal genes in the common ancestor of land plants suggests a key role of mycorrhizas in the colonization of land by plants .
| | Author: Wang B Yeun LH Xue JY Liu Y Ane JM Qiu YL | | Journal: New Phytol Citation: V : 186 P : 514-25 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20059702 Accession (PMID): 20059702 | | Abstract: *The colonization of land by plants fundamentally altered environmental conditions on earth .
Plant-mycorrhizal fungus symbiosis likely played a key role in this process by assisting plants to absorb water and nutrients from soil .
*Here , in a diverse set of land plants , we investigated the evolutionary histories and functional conservation of three genes required for mycorrhiza formation in legumes and rice ( Oryza sativa ) , DMI1 , DMI3 and IPD3 .
*The genes were isolated from nearly all major plant lineages .
Phylogenetic analyses showed that they had been vertically inherited since the origin of land plants .
Further , cross-species mutant rescue experiments demonstrated that DMI3 genes from liverworts and hornworts could rescue Medicago truncatula dmi3 mutants for mycorrhiza formation .
Yeast two-hybrid assays also showed that bryophyte DMI3 proteins could bind to downstream-acting M trunculata IPD3 protein .
Finally , molecular evolutionary analyses revealed that these genes were under purifying selection for maintenance of their ancestral functions in all mycorrhizal plant lineages .
*These results indicate that the mycorrhizal genes were present in the common ancestor of land plants , and that their functions were largely conserved during land plant evolution .
The evidence presented here strongly suggests that plant-mycorrhizal fungus symbiosis was one of the key processes that contributed to the origin of land flora .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: *Here , in a diverse set of land plants , we investigated the evolutionary histories and functional conservation of three genes required for mycorrhiza formation in legumes and rice ( Oryza sativa ) , DMI1 , DMI3 and IPD3 .
[ Sen. 7, subscore: 1.00 ]: Yeast two-hybrid assays also showed that bryophyte DMI3 proteins could bind to downstream-acting M trunculata IPD3 protein .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: OsIPD3 , an ortholog of the Medicago truncatula DMI3 interacting protein IPD3 , is required for mycorrhizal symbiosis in rice .
| | Author: Chen C Ane JM Zhu H | | Journal: New Phytol Citation: V : 180 P : 311-5 Year: 2008 Type: In-Process | | Literature: oryza Field: title Doc ID: pub18761634 Accession (PMID): 18761634 | | Abstract: Medicago truncatula IPD3 ( MtIPD3 ) is an interacting protein of DMI3 ( does not make infections 3 ) , a Ca ( 2+ ) /calmodulin-dependent protein kinase ( CCaMK ) essential for both arbuscular mycorrhizal ( AM ) and rhizobial symbioses .
However , the function of MtIPD3 in root symbioses has not been demonstrated in M truncatula , because of a lack of knockout mutants for functional analysis .
In this study , the availability of IPD3 knockout mutants in rice ( Oryza sativa ) was exploited to test the function of OsIPD3 in AM symbiosis .
Three independent retrotransposon Tos17 insertion lines of OsIPD3 were selected and the phenotypes characterized upon inoculation with the AM fungus Glomus intraradices .
Phenotypic and genetic analyses revealed that the Osipd3 mutants were unable to establish a symbiotic association with G intraradices .
In conclusion , IPD3 represents a novel gene required for root symbiosis with AM fungi in plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: OsIPD3 , an ortholog of the Medicago truncatula DMI3 interacting protein IPD3 , is required for mycorrhizal symbiosis in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
