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Score: 7.00 |
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| Title: The novel gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of rice encodes a putative coiled-coil protein required for homologous chromosome pairing in meiosis .
| | Author: Nonomura K Nakano M Fukuda T Eiguchi M Miyao A Hirochika H Kurata N | | Journal: Plant Cell Citation: V : 16 ( 4 ) P : 1008-20 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15031413 Accession (PMID): 15031413 | | Abstract: We have identified and characterized a novel gene , PAIR1 ( HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 ) , required for homologous chromosome pairing and cytokinesis in male and female meiocytes of rice ( Oryza sativa ) .
The pair1 mutation , tagged by the endogenous retrotransposon Tos17 , exhibited meiosis-specific defects and resulted in complete sterility in male and female gametes .
The PAIR1 gene encodes a 492-amino acid protein , which contains putative coiled-coil motifs in the middle , two basic regions at both termini , and a potential nuclear localization signal at the C terminus .
Expression of the PAIR1 gene was detected in the early stages of flower development , in which the majority of the sporocytes had not entered meiosis .
During prophase I of the pair1 meiocyte , all the chromosomes became entangled to form a compact sphere adhered to a nucleolus , and homologous pairing failed .
At anaphase I and telophase I , chromosome nondisjunction and degenerated spindle formation resulted in multiple uneven spore production .
However , chromosomal fragmentation frequent in plant meiotic mutants was never observed in all of the pair1 meiocytes .
These observations clarify that the PAIR1 protein plays an essential role in establishment of homologous chromosome pairing in rice meiosis . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have identified and characterized a novel gene , PAIR1 ( HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 ) , required for homologous chromosome pairing and cytokinesis in male and female meiocytes of rice ( Oryza sativa ) .
[ Sen. 2, subscore: 1.00 ]: The pair1 mutation , tagged by the endogenous retrotransposon Tos17 , exhibited meiosis-specific defects and resulted in complete sterility in male and female gametes .
[ Sen. 3, subscore: 1.00 ]: The PAIR1 gene encodes a 492-amino acid protein , which contains putative coiled-coil motifs in the middle , two basic regions at both termini , and a potential nuclear localization signal at the C terminus .
[ Sen. 4, subscore: 1.00 ]: Expression of the PAIR1 gene was detected in the early stages of flower development , in which the majority of the sporocytes had not entered meiosis .
[ Sen. 5, subscore: 1.00 ]: During prophase I of the pair1 meiocyte , all the chromosomes became entangled to form a compact sphere adhered to a nucleolus , and homologous pairing failed .
[ Sen. 7, subscore: 1.00 ]: However , chromosomal fragmentation frequent in plant meiotic mutants was never observed in all of the pair1 meiocytes .
[ Sen. 8, subscore: 1.00 ]: These observations clarify that the PAIR1 protein plays an essential role in establishment of homologous chromosome pairing in rice meiosis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: The rice gene DEFECTIVE TAPETUM AND MEIOCYTES 1 ( DTM1 ) is required for early tapetum development and meiosis .
| | Author: Yi J Kim SR Lee DY Moon S Lee YS Jung KH Hwang I An G | | Journal: Plant J Citation: V : 70 P : 256-70 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22111585 Accession (PMID): 22111585 | | Abstract: Tapetum development and meiosis play crucial roles in anther development .
Here we identified a rice gene , DEFECTIVE TAPETUM AND MEIOCYTES 1 ( DTM1 ) , which controls the early stages of that development .
This gene encodes for an endoplasmic reticulum ( ER ) membrane protein that is present only in cereals .
Our T-DNA insertion mutations gave rise to abnormal tapetal formation .
Cellular organelles , especially the ER , were underdeveloped , which led to hampered differentiation and degeneration of the tapetum .
In addition , the development of pollen mother cells was arrested at the early stages of meiotic prophase I RNA in-situ hybridization analyses showed that DTM1 transcripts were most abundant in tapetal cells at stages 6 and 7 , and moderately in the pollen mother cells and meiocytes .
Transcripts of UDT1 , which functions in tapetum development during early meiosis , were reduced in dtm1 anthers , as were those of PAIR1 , which is involved in chromosome pairing and synapsis during meiosis .
However , expression of MSP1 and MEL1 , which function in anther wall specification and germ cell division , respectively , was not altered in the dtm1 mutant .
Moreover , transcripts of DTM1 were reduced in msp1 mutant anthers , but not in udt1 and pair1 mutants .
These results , together with their mutant phenotypes , suggest that DTM1 plays important roles in the ER membrane during early tapetum development , functioning after MSP1 and before UDT1 , and also in meiocyte development , after MEL1 and before PAIR1 .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Transcripts of UDT1 , which functions in tapetum development during early meiosis , were reduced in dtm1 anthers , as were those of PAIR1 , which is involved in chromosome pairing and synapsis during meiosis .
[ Sen. 9, subscore: 1.00 ]: Moreover , transcripts of DTM1 were reduced in msp1 mutant anthers , but not in udt1 and pair1 mutants .
[ Sen. 10, subscore: 1.00 ]: These results , together with their mutant phenotypes , suggest that DTM1 plays important roles in the ER membrane during early tapetum development , functioning after MSP1 and before UDT1 , and also in meiocyte development , after MEL1 and before PAIR1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Global gene profiling of laser-captured pollen mother cells indicates molecular pathways and gene subfamilies involved in rice meiosis .
| | Author: Tang X Zhang ZY Zhang WJ Zhao XM Li X Zhang D Liu QQ Tang WH | | Journal: Plant Physiol Citation: V : 154 P : 1855-70 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20959420 Accession (PMID): 20959420 | | Abstract: Pollen mother cells ( PMCs ) represent a critical early stage in plant sexual reproduction in which the stage is set for male gamete formation .
Understanding the global molecular genetics of this early meiotic stage has so far been limited to whole stamen or floret transcriptome studies , but since PMCs are a discrete population of cells in developmental synchrony , they provide the potential for precise transcriptome analysis and for enhancing our understanding of the transition to meiosis .
As a step toward identifying the premeiotic transcriptome , we performed microarray analysis on a homogenous population of rice ( Oryza sativa ) PMCs isolated by laser microdissection and compared them with those of tricellular pollen and seedling .
Known meiotic genes , including OsSPO11-1 , PAIR1 , PAIR2 , PAIR3 , OsDMC1 , OsMEL1 , OsRAD21-4 , OsSDS , and ZEP1 , all showed preferential expression in PMCs .
The Kyoto Encyclopedia of Genes and Genomes pathways significantly enriched in PMC-preferential genes are DNA replication and repair pathways .
Our genome-wide survey showed that , in the buildup to meiosis , PMCs accumulate the molecular machinery for meiosis at the mRNA level .
We identified 1 , 158 PMC-preferential genes and suggested candidate genes and pathways involved in meiotic recombination and meiotic cell cycle control .
Regarding the developmental context for meiosis , the DEF-like , AGL2-like , and AGL6-like subclades of MADS box transcription factors are PMC-preferentially expressed , the trans-zeatin type of cytokinin might be preferentially synthesized , and the gibberellin signaling pathway is likely active in PMCs .
The ubiquitin-mediated proteolysis pathway is enriched in the 127 genes that are expressed in PMCs but not in tricellular pollen or seedling .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Known meiotic genes , including OsSPO11-1 , PAIR1 , PAIR2 , PAIR3 , OsDMC1 , OsMEL1 , OsRAD21-4 , OsSDS , and ZEP1 , all showed preferential expression in PMCs .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
