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| Title: Isolation and characterization of a beta-tubulin-encoding gene from Colletotrichum gloeosporioides f . sp . aeschynomene .
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| Author: Buhr TL Dickman MB .
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| Journal: Gene Citation: V : 124 ( 1 ) P : 121-5 Year: 1993 Type: ARTICLE |
| Literature: oryza Field: abstract Doc ID: pub8440473 Accession (PMID): 8440473 |
| Abstract: Colletotrichum gloeosporioides f . sp . aeschynomene ( Cg . a . ) is a fungal pathogen of legumes and is used as a commercial mycoherbicide for rice and soybeans .
As an initial study to potentially improve the utility of this fungus and develop a gene transfer system , a beta-tubulin ( beta Tub ) -encoding gene ( TUB1 ) was isolated , cloned and sequenced .
The coding sequence and deduced amino acid sequence of the Cg . a .
TUB1 gene was highly homologous to the TUB1 gene of Colletotrichum graminicola .
Southern hybridizations , using the Cg . a .
TUB1 and C graminicola TUB2 genes as probes , suggest that Cg . a . contains two TUB genes .
Variation in both the restriction pattern and the number of TUB genes present in different formae specialis of C gloeosporioides was evident .
These observations are relevant for assessing relationships among formae specialis of C gloeosporioides .
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[ Sen. 6, subscore: 1.00 ]: TUB1 and C graminicola TUB2 genes as probes , suggest that Cg . a . contains two TUB genes .
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