Score: 7.00 |
|---|
| Title: Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance .
| | Author: Wang YS Pi LY Chen X Chakrabarty PK Jiang J De Leon AL Liu GZ Li L Benny U Oard J Ronald PC Song WY .
| | Journal: Citation: V : ( ) P : Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17172358 Accession (PMID): 17172358 | | Abstract: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae .
We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase .
The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays .
XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively .
Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae .
Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein .
These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . | Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays .
[ Sen. 2, subscore: 1.00 ]: We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase .
[ Sen. 4, subscore: 1.00 ]: XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively .
[ Sen. 5, subscore: 1.00 ]: Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae .
[ Sen. 6, subscore: 1.00 ]: Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein .
[ Sen. 7, subscore: 1.00 ]: These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
