| 19 matches found in 6 documents. Search time: 0.773 seconds. |
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Score: 10.00 |
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| Title: Hexokinase-mediated sugar signaling controls expression of the calcineurin B-like interacting protein kinase 15 gene and is perturbed by oxidative phosphorylation inhibition .
| | Author: Yim HK Lim MN Lee SE Lim J Lee Y Hwang YS | | Journal: J Plant Physiol Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22796010 Accession (PMID): 22796010 | | Abstract: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice .
It is located upstream and probably contributes to reserve mobilization under anoxia .
In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression .
Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 .
For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not .
By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene .
Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 .
This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status .
Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control .
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[ Sen. 9, subscore: 3.00 ]: Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control .
[ Sen. 3, subscore: 2.00 ]: In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression .
[ Sen. 1, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice .
[ Sen. 4, subscore: 1.00 ]: Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 .
[ Sen. 5, subscore: 1.00 ]: For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not .
[ Sen. 6, subscore: 1.00 ]: By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene .
[ Sen. 7, subscore: 1.00 ]: Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 .
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Score: 7.00 |
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| Title: Regulatory interplay of the Sub1A and CIPK15 pathways in the regulation of alpha-amylase production in flooded rice plants .
| | Author: Kudahettige NP Pucciariello C Parlanti S Alpi A Perata P | | Journal: Plant Biol ( Stuttg ) Citation: V : 13 P : 611-9 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21668602 Accession (PMID): 21668602 | | Abstract: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded .
Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties .
Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants .
It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation .
In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator .
In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated .
The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety .
CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A .
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[ Sen. 5, subscore: 2.00 ]: In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator .
[ Sen. 3, subscore: 1.00 ]: Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants .
[ Sen. 4, subscore: 1.00 ]: It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation .
[ Sen. 6, subscore: 1.00 ]: In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated .
[ Sen. 7, subscore: 1.00 ]: The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety .
[ Sen. 8, subscore: 1.00 ]: CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A .
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Score: 2.00 |
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| Title: Coordinated responses to oxygen and sugar deficiency allow rice seedlings to tolerate flooding .
| | Author: Lee KW Chen PW Lu CA Chen S Ho TH Yu SM | | Journal: Sci Signal Citation: V : 2 P : ra61 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19809091 Accession (PMID): 19809091 | | Abstract: Flooding is a widespread natural disaster that leads to oxygen ( O ( 2 ) ) and energy deficiency in terrestrial plants , thereby reducing their productivity .
Rice is unusually tolerant to flooding , but the underlying mechanism for this tolerance has remained elusive .
Here , we show that protein kinase CIPK15 [ calcineurin B-like ( CBL ) -interacting protein kinase ] plays a key role in O ( 2 ) -deficiency tolerance in rice .
CIPK15 regulates the plant global energy and stress sensor SnRK1A ( Snf1-related protein kinase 1 ) and links O ( 2 ) -deficiency signals to the SnRK1-dependent sugar-sensing cascade to regulate sugar and energy production and to enable rice growth under floodwater .
Our studies contribute to understanding how rice grows under the conditions of O ( 2 ) deficiency necessary for growing rice in irrigated lowlands .
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[ Sen. 3, subscore: 1.00 ]: Here , we show that protein kinase CIPK15 [ calcineurin B-like ( CBL ) -interacting protein kinase ] plays a key role in O ( 2 ) -deficiency tolerance in rice .
[ Sen. 4, subscore: 1.00 ]: CIPK15 regulates the plant global energy and stress sensor SnRK1A ( Snf1-related protein kinase 1 ) and links O ( 2 ) -deficiency signals to the SnRK1-dependent sugar-sensing cascade to regulate sugar and energy production and to enable rice growth under floodwater .
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Score: 2.00 |
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| Title: New regulator for energy signaling pathway in plants highlights conservation among species .
| | Author: Meyer AK Longin CF Klose C Hermann A | | Journal: Sci Signal Citation: V : 3 P : jc5 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20424261 Accession (PMID): 20424261 | | Abstract: The "low-energy checkpoint" SNF1-related protein kinases , which are conserved in all eukaryotes , play an important role in cellular metabolic adaptation to differences in energy and oxygen availability .
Although the signaling pathways involved in such metabolic adaptations are well understood in yeast and mammals , they have been poorly understood in plants .
A recent study revealed that calcineurin B-like interacting protein kinase 15 ( CIPK15 ) acted as a global regulator of such adaptations , linking the response to O ( 2 ) deficiency with the response to carbohydrate starvation in rice ( Oryza sativa ) .
Knockout mutants of Nipponbare rice CIPK15 failed to initiate transcription of the glycolytic enzymes alpha-amylase 3 and alcohol dehydrogenase 2 , which mediate fermentative metabolism for adenosine triphosphate generation under anaerobic conditions .
Targeted manipulation of OsCIPK15 might facilitate rice cultivation and ensure agricultural productivity in regions subject to flooding .
Here , we highlight the importance of the energy and oxygen-sensing pathway indicated by its conservation among different eukaryotic kingdoms .
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[ Sen. 3, subscore: 1.00 ]: A recent study revealed that calcineurin B-like interacting protein kinase 15 ( CIPK15 ) acted as a global regulator of such adaptations , linking the response to O ( 2 ) deficiency with the response to carbohydrate starvation in rice ( Oryza sativa ) .
[ Sen. 4, subscore: 1.00 ]: Knockout mutants of Nipponbare rice CIPK15 failed to initiate transcription of the glycolytic enzymes alpha-amylase 3 and alcohol dehydrogenase 2 , which mediate fermentative metabolism for adenosine triphosphate generation under anaerobic conditions .
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Score: 1.00 |
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| Title: Interference with oxidative phosphorylation enhances anoxic expression of rice alpha-amylase genes through abolishing sugar regulation .
| | Author: Park M Yim HK Park HG Lim J Kim SH Hwang YS | | Journal: J Exp Bot Citation: V : 61 P : 3235-44 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20530196 Accession (PMID): 20530196 | | Abstract: Rice has the unique ability to express alpha-amylase under anoxic conditions , a feature that is critical for successful anaerobic germination and growth .
Previously , anaerobic conditions were shown to up-regulate the expression of Amy3 subfamily genes ( Amy3B/C , 3D , and 3E ) in rice embryos .
These genes are known to be feedback regulated by the hydrolytic products of starchy endosperm such as the simple sugar glucose .
It was found that oxygen deficiency interferes with the repression of Amy3D gene expression imposed by low concentrations of glucose but not with that imposed by higher amounts .
This differential anoxic de-repression depending on sugar concentration suggests the presence of two distinct pathways for sugar regulation of Amy3D gene expression .
Anoxic de-repression can be mimicked by treating rice embryos with inhibitors of ATP synthesis during respiration .
Other sugar-regulated rice alpha-amylase genes , Amy3B/C and 3E , behave similarly to Amy3D .
Treatment with a respiratory inhibitor or anoxia also relieved the sugar repression of the rice CIPK15 gene , a main upstream positive regulator of SnRK1A that is critical for Amy3D expression in response to sugar starvation .
SnRK1A accumulation was previously shown to be required for MYBS1 expression , which transactivates Amy3D by binding to a cis-acting element found in the proximal region of all Amy3 subfamily gene promoters ( the TA box ) .
Taken together , these results suggest that prevention of oxidative phosphorylation by oxygen deficiency interferes with the sugar repression of Amy3 subfamily gene expression , leading to their enhanced expression in rice embryos during anaerobic germination .
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[ Sen. 8, subscore: 1.00 ]: Treatment with a respiratory inhibitor or anoxia also relieved the sugar repression of the rice CIPK15 gene , a main upstream positive regulator of SnRK1A that is critical for Amy3D expression in response to sugar starvation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
