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Title: Functional characterization of a putative nitrate transporter gene promoter from rice .
Author: Hu TZ Cao KM Xia M Wang XP .
Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 38 ( 11 ) P : 795-802 Year: 2006 Type: ARTICLE
Literature: oryza Field: abstract Doc ID: pub17091197 Accession (PMID): 17091197
Abstract: Drought is one of the most significant abiotic stresses that influence plant growth and development . Expression analysis revealed that OsNRT1 . 3 , a putative nitrate transporter gene in rice , was induced by drought . To confirm if the OsNRT1 . 3 promoter can respond to drought stress , a 2019 bp upstream sequence of OsNRT1 . 3 was cloned . Three OsNRT1 . 3 promoter fragments were generated by 5-deletion , and fused to the beta-glucuronidase ( GUS ) gene . The chimeric genes were introduced into rice plants . NRT2019 : : GUS , NRT1196 : : GUS and NRT719 : : GUS showed similar expression patterns in seeds , roots , leaves and flowers in all transgenic rice , and GUS activity conferred by different OsNRT1 . 3 promoter fragments was significantly upregulated by drought stress , indicating that OsNRT1 . 3 promoter responds to drought stress and the 719 bp upstream sequence of OsNRT1 . 3 contains the drought response elements .
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[ Sen. 6, subscore: 1.00 ]: NRT2019 : : GUS , NRT1196 : : GUS and NRT719 : : GUS showed similar expression patterns in seeds , roots , leaves and flowers in all transgenic rice , and GUS activity conferred by different OsNRT1 . 3 promoter fragments was significantly upregulated by drought stress , indicating that OsNRT1 . 3 promoter responds to drought stress and the 719 bp upstream sequence of OsNRT1 . 3 contains the drought response elements .
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