| 136 matches found in 59 documents. Search time: 0.31 seconds. |
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Score: 13.00 |
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| Title: Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion genes in transgenic rice .
| | Author: Kyozuka J McElroy D Hayakawa T Xie Y Wu R Shimamoto K | | Journal: Plant Physiol .
Citation: V : 102 ( 3 ) P : 991-1000 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8278540 Accession (PMID): 8278540 | | Abstract: A previously isolated rice ( Oryza sativa ) rbcS gene was further characterized .
This analysis revealed specific sequences in the 5 regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species .
In transgenic rice plants , we examined the expression of the beta-glucuronidase ( gusA ) reporter gene directed by the 2 . 8-kb promoter region of the rice rbcS gene .
To examine differences in the regulation of monocotyledonous and dicotyledonous rbcS promoters , the activity of a tomato rbcS promoter was also investigated in transgenic rice plants .
Our results indicated that both rice and tomato rbcS promoters confer mesophyll-specific expression of the gusA reporter gene in transgenic rice plants and that this expression is induced by light .
However , the expression level of the rice rbcS-gusA gene was higher than that of the tomato rbcS-gusA gene , suggesting the presence of quantitative differences in the activity of these particular monocotyledonous and dicotyledonous rbcS promoters in transgenic rice .
Histochemical analysis of rbcS-gusA gene expression showed that the observed light induction was only found in mesophyll cells .
Furthermore , it was demonstrated that the light regulation of rice rbcS-gusA gene expression was primarily at the level of mRNA accumulation .
We show that the rice rbcS gene promoter should be useful for expression of agronomically important genes for genetic engineering of monocotyledonous species . | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: However , the expression level of the rice rbcS-gusA gene was higher than that of the tomato rbcS-gusA gene , suggesting the presence of quantitative differences in the activity of these particular monocotyledonous and dicotyledonous rbcS promoters in transgenic rice .
[ Sen. 2, subscore: 2.00 ]: This analysis revealed specific sequences in the 5 regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species .
[ Sen. 4, subscore: 2.00 ]: To examine differences in the regulation of monocotyledonous and dicotyledonous rbcS promoters , the activity of a tomato rbcS promoter was also investigated in transgenic rice plants .
[ Sen. 1, subscore: 1.00 ]: A previously isolated rice ( Oryza sativa ) rbcS gene was further characterized .
[ Sen. 3, subscore: 1.00 ]: In transgenic rice plants , we examined the expression of the beta-glucuronidase ( gusA ) reporter gene directed by the 2 . 8-kb promoter region of the rice rbcS gene .
[ Sen. 5, subscore: 1.00 ]: Our results indicated that both rice and tomato rbcS promoters confer mesophyll-specific expression of the gusA reporter gene in transgenic rice plants and that this expression is induced by light .
[ Sen. 7, subscore: 1.00 ]: Histochemical analysis of rbcS-gusA gene expression showed that the observed light induction was only found in mesophyll cells .
[ Sen. 8, subscore: 1.00 ]: Furthermore , it was demonstrated that the light regulation of rice rbcS-gusA gene expression was primarily at the level of mRNA accumulation .
[ Sen. 9, subscore: 1.00 ]: We show that the rice rbcS gene promoter should be useful for expression of agronomically important genes for genetic engineering of monocotyledonous species .
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Score: 10.00 |
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| Title: Availability of Rubisco Small Subunit Up-Regulates the Transcript Levels of Large Subunit for Stoichiometric Assembly of Its Holoenzyme in Rice .
| | Author: Suzuki Y Makino A | | Journal: Plant Physiol Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22811433 Accession (PMID): 22811433 | | Abstract: Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome .
For synthesis of the Rubisco holoenzyme , both genes need to be expressed coordinately .
To investigate this molecular mechanism , protein synthesis of two subunits of Rubisco was characterized in transgenic rice ( Oryza sativa L ) plants with overexpression or antisense suppression of RBCS gene .
Total RBCS and rbcL mRNA levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants , although an increase in total RBCS mRNA level was greater .
In RBCS-antisense plants , the levels of these mRNAs and synthesis of the corresponding proteins declined to a similar extent .
The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes .
The level of rbcL mRNA in turn was tightly correlated with the amount of RbcL synthesized , the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype .
Polysome loading of rbcL mRNA was not changed .
These results demonstrate that the availability of RBCS protein up-regulates gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme .
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[ Sen. 4, subscore: 4.00 ]: Total RBCS and rbcL mRNA levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants , although an increase in total RBCS mRNA level was greater .
[ Sen. 1, subscore: 1.00 ]: Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome .
[ Sen. 3, subscore: 1.00 ]: To investigate this molecular mechanism , protein synthesis of two subunits of Rubisco was characterized in transgenic rice ( Oryza sativa L ) plants with overexpression or antisense suppression of RBCS gene .
[ Sen. 5, subscore: 1.00 ]: In RBCS-antisense plants , the levels of these mRNAs and synthesis of the corresponding proteins declined to a similar extent .
[ Sen. 6, subscore: 1.00 ]: The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes .
[ Sen. 7, subscore: 1.00 ]: The level of rbcL mRNA in turn was tightly correlated with the amount of RbcL synthesized , the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype .
[ Sen. 9, subscore: 1.00 ]: These results demonstrate that the availability of RBCS protein up-regulates gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme .
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Score: 8.00 |
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| Title: Developmental characteristics and aluminum resistance of root border cells in rice seedlings .
| | Author: Cai M Zhang S Xing C Wang F Ning W Lei Z | | Journal: Plant Sci Citation: V : 180 P : 702-8 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21421421 Accession (PMID): 21421421 | | Abstract: The developmental characteristics of root border cells ( RBCs ) and their role in protection of root apices of rice seedling from Al toxicity were evaluated in two rice ( Oryza sativa L ) cultivars differing in Al tolerance .
Root elongation and RBCs viability were used as indicators for Al effects .
The formation of RBCs and the emergence of the root tip occurred almost simultaneously .
Treatment of the root with Al inhibited root elongation and increased Al accumulation in the root tips .
Physical removal of RBCs from root tips resulted in a more severe inhibition of root elongation and a higher Al accumulation in the root tips .
These effects were more pronounced in the Al-sensitive rice cultivar ( II You 6216 ) than that in the Al-tolerant rice cultivar ( II You 838 ) .
The relative viability of attached and detached RBCs decreased with increasing Al concentrations .
Al also induced a thicker mucilage layer surrounding attached RBCs of both cultivars , and detached RBCs did not .
Maintaining the abundant live RBCs encapsulated root tip and enhancing their mucilage secretion , appear to be important in alleviating Al toxicity and in allowing exclusion of Al from the rice root apex | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Al also induced a thicker mucilage layer surrounding attached RBCs of both cultivars , and detached RBCs did not .
[ Sen. 1, subscore: 1.00 ]: The developmental characteristics of root border cells ( RBCs ) and their role in protection of root apices of rice seedling from Al toxicity were evaluated in two rice ( Oryza sativa L ) cultivars differing in Al tolerance .
[ Sen. 2, subscore: 1.00 ]: Root elongation and RBCs viability were used as indicators for Al effects .
[ Sen. 3, subscore: 1.00 ]: The formation of RBCs and the emergence of the root tip occurred almost simultaneously .
[ Sen. 5, subscore: 1.00 ]: Physical removal of RBCs from root tips resulted in a more severe inhibition of root elongation and a higher Al accumulation in the root tips .
[ Sen. 7, subscore: 1.00 ]: The relative viability of attached and detached RBCs decreased with increasing Al concentrations .
[ Sen. 9, subscore: 1.00 ]: Maintaining the abundant live RBCs encapsulated root tip and enhancing their mucilage secretion , appear to be important in alleviating Al toxicity and in allowing exclusion of Al from the rice root apex
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Score: 8.00 |
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| Title: Effect of individual suppression of RBCS multigene family on Rubisco contents in rice leaves .
| | Author: Ogawa S Suzuki Y Yoshizawa R Kanno K Makino A | | Journal: Plant Cell Environ Citation: V : 35 P : 546-53 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21951138 Accession (PMID): 21951138 | | Abstract: In higher plants , a small subunit of Rubisco is encoded for by an RBCS multigene family in the nuclear genome .
However , it is unknown how each multigene member contributes to the accumulation of Rubisco holoenzyme .
Here , four RBCS genes that are highly expressed in leaf blaedes of rice ( Oryza sativa L ) were individually suppressed by RNAi , and the effects on leaf Rubisco content were examined at seedling , vegetative and reproductive stages .
Rubisco contents in each transgenic line declined irrespective of growth stage , and the ratios of Rubisco-N to total N were 66-96% of wild-type levels .
The mRNA levels of the suppressed RBCS genes declined significantly , whereas those of the unsuppressed ones did not change drastically .
These results indicate that four RBCS genes all contribute to accumulation of Rubisco holoenzyme irrespective of growth stage and that suppression of one RBCS gene is not fully compensated by other RBCS genes .
Additionally , the mRNA levels of the large subunit of Rubisco showed a change similar to that of total RBCS mRNA level irrespective of genotype and growth stage .
These results suggest that gene expression of RBCS and RBCL is regulated in a coordinated manner at the transcript level in rice .
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[ Sen. 6, subscore: 3.00 ]: These results indicate that four RBCS genes all contribute to accumulation of Rubisco holoenzyme irrespective of growth stage and that suppression of one RBCS gene is not fully compensated by other RBCS genes .
[ Sen. 1, subscore: 1.00 ]: In higher plants , a small subunit of Rubisco is encoded for by an RBCS multigene family in the nuclear genome .
[ Sen. 3, subscore: 1.00 ]: Here , four RBCS genes that are highly expressed in leaf blaedes of rice ( Oryza sativa L ) were individually suppressed by RNAi , and the effects on leaf Rubisco content were examined at seedling , vegetative and reproductive stages .
[ Sen. 5, subscore: 1.00 ]: The mRNA levels of the suppressed RBCS genes declined significantly , whereas those of the unsuppressed ones did not change drastically .
[ Sen. 7, subscore: 1.00 ]: Additionally , the mRNA levels of the large subunit of Rubisco showed a change similar to that of total RBCS mRNA level irrespective of genotype and growth stage .
[ Sen. 8, subscore: 1.00 ]: These results suggest that gene expression of RBCS and RBCL is regulated in a coordinated manner at the transcript level in rice .
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Score: 7.00 |
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| Title: The promoter of rbcS in a C3 plant ( rice ) directs organ-specific , light-dependent expression in a C4 plant ( maize ) , but does not confer bundle sheath cell-specific expression .
| | Author: Nomura M Katayama K Nishimura A Ishida Y Ohta S Komari T Miyao-Tokutomi M Tajima S Matsuoka M | | Journal: Plant Mol . Biol . Citation: V : 44 ( 1 ) P : 99-106 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11094984 Accession (PMID): 11094984 | | Abstract: The small subunit of ribulose-bisphosphate carboxylase ( Rubisco ) , encoded by rbcS , is essential for photosynthesis in both C3 and C4 plants , even though the cell specificity of rbcS expression is different between C3 and C4 plants .
The C3 rbcS is specifically expressed in mesophyll cells , while the C4 rbcS is expressed in bundle sheath cells , and not mesophyll cells .
Two chimeric genes were constructed consisting of the structural gene encoding beta-glucuronidase ( GUS ) controlled by the two promoters from maize ( C4 ) and rice ( C3 ) rbcS genes .
These constructs were introduced into a C4 plant , maize .
Both chimeric genes were specifically expressed in photosynthetic organs , such as leaf blade , but not in non-photosynthetic organs .
The expressions of the genes were also regulated by light .
However , the rice promoter drove the GUS activity mainly in mesophyll cells and relatively low in bundle sheath cells , while the maize rbcS promoter induced the activity specifically in bundle sheath cells .
These results suggest that the rice promoter contains some cis-acting elements responding in an organ-specific and light-inducible regulation manner in maize but does not contain element ( s ) for bundle sheath cell-specific expression , while the maize promoter does contain such element ( s ) .
Based on this result , we discuss the similarities and differences between the rice ( C3 ) and maize ( C4 ) rbcS promoter in terms of the evolution of the C4 photosynthetic gene . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The small subunit of ribulose-bisphosphate carboxylase ( Rubisco ) , encoded by rbcS , is essential for photosynthesis in both C3 and C4 plants , even though the cell specificity of rbcS expression is different between C3 and C4 plants .
[ Sen. 2, subscore: 2.00 ]: The C3 rbcS is specifically expressed in mesophyll cells , while the C4 rbcS is expressed in bundle sheath cells , and not mesophyll cells .
[ Sen. 3, subscore: 1.00 ]: Two chimeric genes were constructed consisting of the structural gene encoding beta-glucuronidase ( GUS ) controlled by the two promoters from maize ( C4 ) and rice ( C3 ) rbcS genes .
[ Sen. 7, subscore: 1.00 ]: However , the rice promoter drove the GUS activity mainly in mesophyll cells and relatively low in bundle sheath cells , while the maize rbcS promoter induced the activity specifically in bundle sheath cells .
[ Sen. 9, subscore: 1.00 ]: Based on this result , we discuss the similarities and differences between the rice ( C3 ) and maize ( C4 ) rbcS promoter in terms of the evolution of the C4 photosynthetic gene .
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