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Score: 15.00 |
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| Title: Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 ( Xa5 ) in comparison with its homolog TFIIAgamma1 .
| | Author: Jiang GH Xia ZH Zhou YL Wan J Li DY Chen RS Zhai WX Zhu LH .
| | Journal: Citation: V : ( ) P : 1-13 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16444514 Accession (PMID): 16444514 | | Abstract: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
The rice has an addition gene for TFIIAgamma exists on chromosome 1 ( TFIIAgamma1 ) .
Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
However , no expression of TFIIAgamma1 could be detected in the panicle by reverse transcriptase-polymerase chain reaction .
To compare the structural difference between the Xa5/xa5 and TFIIAgamma1 proteins , 3-D structures were predicted using computer-aided modeling techniques .
The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
| Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
[ Sen. 3, subscore: 3.00 ]: Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
[ Sen. 8, subscore: 2.00 ]: The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
[ Sen. 10, subscore: 2.00 ]: The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
[ Sen. 1, subscore: 1.00 ]: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
[ Sen. 5, subscore: 1.00 ]: Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
[ Sen. 9, subscore: 1.00 ]: The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 15.00 |
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| Title: Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 ( Xa5 ) in comparison with its homolog TFIIAgamma1 .
| | Author: Jiang GH Xia ZH Zhou YL Wan J Li DY Chen RS Zhai WX Zhu LH .
| | Journal: Mol . Genet . Genomics Citation: V : 275 ( 4 ) P : 354-66 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16614777 Accession (PMID): 16614777 | | Abstract: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
The rice has an addition gene for TFIIAgamma exists on chromosome 1 ( TFIIAgamma1 ) .
Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
However , no expression of TFIIAgamma1 could be detected in the panicle by reverse transcriptase-polymerase chain reaction .
To compare the structural difference between the Xa5/xa5 and TFIIAgamma1 proteins , 3-D structures were predicted using computer-aided modeling techniques .
The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively . | Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
[ Sen. 3, subscore: 3.00 ]: Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
[ Sen. 8, subscore: 2.00 ]: The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
[ Sen. 10, subscore: 2.00 ]: The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
[ Sen. 1, subscore: 1.00 ]: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
[ Sen. 5, subscore: 1.00 ]: Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
[ Sen. 9, subscore: 1.00 ]: The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 10.00 |
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| Title: Transcription activator-like type III effector AvrXa27 depends on OsTFIIAgamma5 for the activation of Xa27 transcription in rice that triggers disease resistance to Xanthomonas oryzae pv . oryzae .
| | Author: Gu K Tian D Qiu C Yin Z | | Journal: Mol Plant Pathol Citation: V : 10 P : 829-35 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19849788 Accession (PMID): 19849788 | | Abstract: The transcription activator-like ( TAL ) type III effector AvrXa27 from Xanthomonas oryzae pv . oryzae ( Xoo ) strain PXO99 ( A ) activates the transcription of the host resistance gene Xa27 , which results in disease resistance to bacterial blight ( BB ) in rice .
In this study , we show that AvrXa27-activated Xa27 transcription requires host general transcription factor OsTFIIAgamma5 .
The V39E substitution in OsTFIIAgamma5 , encoded by the recessive resistance gene xa5 in rice , greatly attenuates this activation in xa5 and Xa27 double homozygotes on inoculation with Xa27-incompatible strains .
The xa5 gene also causes attenuation in the induction of Xa27 by AvrXa27 expressed in rice .
The xa5-mediated attenuation of Xa27-mediated resistance to PXO99 ( A ) is recessive .
Intriguingly , xa5-mediated resistance to xa5-incompatible strains is also down-regulated in the xa5 and Xa27 double homozygotes .
In addition , AvrXa27 expressed in planta shows weak virulence activity in the xa5 genetic background and causes enhanced susceptibility of the plants to BB inoculation .
The results suggest that TAL effectors target host general transcription factors to directly manipulate the host transcriptional machinery for virulence and/or avirulence .
The identification of xa5-mediated attenuation of Xa27-mediated resistance to Xoo provides a guideline for breeding resistance to BB when pyramiding xa5 with other resistance genes .
| Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: Intriguingly , xa5-mediated resistance to xa5-incompatible strains is also down-regulated in the xa5 and Xa27 double homozygotes .
[ Sen. 3, subscore: 2.00 ]: The V39E substitution in OsTFIIAgamma5 , encoded by the recessive resistance gene xa5 in rice , greatly attenuates this activation in xa5 and Xa27 double homozygotes on inoculation with Xa27-incompatible strains .
[ Sen. 9, subscore: 2.00 ]: The identification of xa5-mediated attenuation of Xa27-mediated resistance to Xoo provides a guideline for breeding resistance to BB when pyramiding xa5 with other resistance genes .
[ Sen. 4, subscore: 1.00 ]: The xa5 gene also causes attenuation in the induction of Xa27 by AvrXa27 expressed in rice .
[ Sen. 5, subscore: 1.00 ]: The xa5-mediated attenuation of Xa27-mediated resistance to PXO99 ( A ) is recessive .
[ Sen. 7, subscore: 1.00 ]: In addition , AvrXa27 expressed in planta shows weak virulence activity in the xa5 genetic background and causes enhanced susceptibility of the plants to BB inoculation .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: Genetic and functional characterization of the rice bacterial blight disease resistance gene xa5 .
| | Author: Iyer-Pascuzzi AS Jiang H Huang L McCouch SR | | Journal: Phytopathology Citation: V : 98 P : 289-95 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18944079 Accession (PMID): 18944079 | | Abstract: Xanthomonas oryzae pv . oryzae is the causal agent of rice bacterial blight , a destructive rice disease worldwide .
The gene xa5 provides race-specific resistance to X oryzae pv . oryzae , and encodes the small subunit of transcription factor IIA .
How xa5 functions in bacterial blight resistance is not well understood , and its recessive gene action is disputed .
Here we show that xa5 is inherited in a completely recessive manner and the susceptible allele Xa5 is fully dominant .
In accordance with this , bacterial growth in heterozygous and homozygous susceptible lines is not significantly different .
Further , one allele of Xa5 is sufficient to promote disease in previously resistant plants ; additional copies are not predictive of increased lesion length .
Surprisingly , a resistant nearly isogenic line ( NIL ) of an indica variety sustains high levels of bacterial populations compared to the susceptible NIL , yet the resistant plants restrict symptom expression .
In contrast , in japonica NILs , bacterial population dynamics differ in resistant and susceptible genotypes .
However , both resistant indica and japonica plants delay bacterial movement down the leaf .
These results support a model in which xa5-mediated recessive resistance is the result of restricted bacterial movement , but not restricted multiplication .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Here we show that xa5 is inherited in a completely recessive manner and the susceptible allele Xa5 is fully dominant .
[ Sen. 2, subscore: 1.00 ]: The gene xa5 provides race-specific resistance to X oryzae pv . oryzae , and encodes the small subunit of transcription factor IIA .
[ Sen. 3, subscore: 1.00 ]: How xa5 functions in bacterial blight resistance is not well understood , and its recessive gene action is disputed .
[ Sen. 6, subscore: 1.00 ]: Further , one allele of Xa5 is sufficient to promote disease in previously resistant plants ; additional copies are not predictive of increased lesion length .
[ Sen. 10, subscore: 1.00 ]: These results support a model in which xa5-mediated recessive resistance is the result of restricted bacterial movement , but not restricted multiplication .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Inheritance of bacterial blight resistance in the rice cultivar Ajaya and high-resolution mapping of a major QTL associated with resistance .
| | Author: Sujatha K Natarajkumar P Laha GS Mishra B Rao KS Viraktamath BC Kirti PB Hari Y Balachandran SM Rajendrakumar P Ram T Hajira SK Madhav MS Neeraja CN Sundaram RM | | Journal: Genet Res ( Camb ) Citation: V : 93 P : 397-408 Year: 2011 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22189605 Accession (PMID): 22189605 | | Abstract: SummaryThe cultivar Ajaya ( IET 8585 ) exhibits durable broad-spectrum resistance to bacterial blight ( BB ) disease of rice and is widely used as a resistance donor .
The present study was carried out to decipher the genetics of BB resistance in Ajaya and map the gene ( s ) conferring resistance .
Genetic analysis in the F2 indicated a quantitative/additive nature of resistance governed by two loci with equal effects .
Linked marker analysis and allelic tests revealed that one of the resistance genes is xa5 .
Sequence analysis of a 244 bp region of the second exon of the gene-encoding Transcription factor IIAgamma ( the candidate gene for xa5 ) confirmed the presence of xa5 .
Bulked-segregant analysis ( BSA ) revealed the putative location of the two quantitative trait loci ( QTLs ) /genes associated with resistance on chromosomes 5 and 8 .
Composite interval mapping located the first locus on Chr .
5S exactly in the genomic region spanned by xa5 and the second locus ( qtl BBR 8 . 1 ) on Chr .
8L .
Owing to its differential disease reaction with a set of seven hyper-virulent isolates of Xanthomonas oryzae , a map location on Chr .
8L , which was distinct from xa13 and data from allelism tests , the second resistance locus in Ajaya was determined to be novel and was designated as xaAj .
A contig map spanning xaAj was constructed in silico and the genomic region was delimited to a 13 . 5 kb physical interval In silico analysis of the genomic region spanning xaAj identified four putatively expressed candidate genes , one of which could be involved in imparting BB resistance in Ajaya along with xa5 .
| Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Sequence analysis of a 244 bp region of the second exon of the gene-encoding Transcription factor IIAgamma ( the candidate gene for xa5 ) confirmed the presence of xa5 .
[ Sen. 4, subscore: 1.00 ]: Linked marker analysis and allelic tests revealed that one of the resistance genes is xa5 .
[ Sen. 8, subscore: 1.00 ]: 5S exactly in the genomic region spanned by xa5 and the second locus ( qtl BBR 8 . 1 ) on Chr .
[ Sen. 12, subscore: 1.00 ]: A contig map spanning xaAj was constructed in silico and the genomic region was delimited to a 13 . 5 kb physical interval In silico analysis of the genomic region spanning xaAj identified four putatively expressed candidate genes , one of which could be involved in imparting BB resistance in Ajaya along with xa5 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
