Score: 10.00 | Title: Hexokinase-mediated sugar signaling controls expression of the calcineurin B-like interacting protein kinase 15 gene and is perturbed by oxidative phosphorylation inhibition .
| Author: Yim HK Lim MN Lee SE Lim J Lee Y Hwang YS | Journal: J Plant Physiol Citation: V : P : Year: 2012 Type: Publisher | Literature: oryza Field: abstract Doc ID: pub22796010 Accession (PMID): 22796010 | Abstract: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice .
It is located upstream and probably contributes to reserve mobilization under anoxia .
In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression .
Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 .
For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not .
By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene .
Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 .
This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status .
Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control .
| Matching Sentences: [ Sen. 9, subscore: 3.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 3, subscore: 2.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 1, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 4, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 5, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 6, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control . [ Sen. 7, subscore: 1.00 ]: Calcineurin B-like ( CBL ) interacting protein kinase 15 ( CIPK15 ) is a newly identified positive regulator which is critical to directing the O ( 2 ) deficiency signal to the sugar signaling cascade as part of Amy3D ( representative Amy3 gene ) regulation in rice . It is located upstream and probably contributes to reserve mobilization under anoxia . In isolated starving embryos , the temporal pattern of accumulation of CIPK15 transcripts and leaky suppression of this gene suggests that factors other than CIPK15 may also be involved in the regulation of Amy3D expression . Probing of a variety of sugars and sugar analogs has shown that hexokinase mediates the sugar regulation of CIPK15 . For example , hexokinase substrates , such as mannose , 2-deoxyglucose , and other metabolizable sugars , repressed CIPK15 expression , whereas 3-O-methylglucose and 6-deoxyglucose did not . By using glucosamine , a hexokinase inhibitor , to release glucose-dependent CIPK15 suppression , we confirmed that hexokinase mediates regulation of this gene . Chemical inhibitors of mitochondrial electron transfer , proton separation or ATP synthase also effectively abolished sugar-induced repression of CIPK15 . This type of interference , the release from glucose-induced repression of gene expression by inhibition of oxidative phosphorylation , was previously identified for the Amy3D gene , which suggests that hexokinase-mediated sugar signaling may be coordinated with the cellular energy status . Analysis of a transgenic rice cell line harboring the GUS reporter gene under the control of the CIPK15 promoter , and transient expression assay for 3 UTR of the CIPK15 gene indicate that sugar regulation of the rice CIPK15 gene is likely mediated by 2548-bp 5-flanking region , with no additional post-transcriptional control .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 | Title: Regulatory interplay of the Sub1A and CIPK15 pathways in the regulation of alpha-amylase production in flooded rice plants .
| Author: Kudahettige NP Pucciariello C Parlanti S Alpi A Perata P | Journal: Plant Biol ( Stuttg ) Citation: V : 13 P : 611-9 Year: 2011 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub21668602 Accession (PMID): 21668602 | Abstract: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded .
Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties .
Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants .
It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation .
In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator .
In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated .
The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety .
CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A .
| Matching Sentences: [ Sen. 5, subscore: 2.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A . [ Sen. 3, subscore: 1.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A . [ Sen. 4, subscore: 1.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A . [ Sen. 6, subscore: 1.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A . [ Sen. 7, subscore: 1.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A . [ Sen. 8, subscore: 1.00 ]: Rice ( Oryza sativa L ) can successfully germinate and grow even when flooded . Rice varieties possessing the submergence 1A ( Sub1A ) gene display a distinct flooding-tolerant phenotype , associated with lower carbohydrate consumption and restriction of the fast-elongation phenotype typical of flooding-intolerant rice varieties . Calcineurin B-like interacting protein kinase 15 ( CIPK15 ) was recently indicated as a key regulator of alpha-amylases under oxygen deprivation , linked to both rice germination and flooding tolerance in adult plants . It is still unknown whether the Sub1A and CIPK15-mediated pathways act as complementary processes for rice survival under O ( 2 ) deprivation . In adult plants Sub1A and CIPK15 may perhaps play an antagonistic role in terms of carbohydrate consumption , with Sub1A acting as a starch degradation repressor and CIPK15 as an activator . In this study , we analysed sugar metabolism in the stem of rice plants under water submergence by selecting cultivars with different traits associated with flooding survival The relation between the Sub1A and the CIPK15 pathways was investigated . The results show that under O ( 2 ) deprivation , the CIPK15 pathway is repressed in the tolerant , Sub1A-containing , FR13A variety . CIPK15 is likely to play a role in the up-regulation of Ramy3D in flooding-intolerant rice varieties that display fast elongation under flooding and that do not possess Sub1A .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |