37 matches found in 13 documents. Search time: 0.002 seconds. |
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Score: 12.00 | Title: Cloning and characterization of barley caryopsis FCA .
| Author: Kumar S Jiang S Jami SK Hill RD | Journal: Physiol Plant Citation: V : 143 P : 93-106 Year: 2011 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub21645000 Accession (PMID): 21645000 | Matching Sentences: [ Sen. 6, subscore: 2.00 ]: FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) . We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv . McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . [ Sen. 10, subscore: 2.00 ]: Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation . [ Sen. 11, subscore: 2.00 ]: In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation . [ Sen. 1, subscore: 1.00 ]: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis . FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) . We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv . McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . [ Sen. 2, subscore: 1.00 ]: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis . FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) . We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv . McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . [ Sen. 3, subscore: 1.00 ]: The RNA binding protein , flowering control locus A , ( FCA ) regulates flowering in rice and Arabidopsis . FCA interacts with FY to auto-regulate its own transcripts as well as to control flowering by downregulating flowering locus C ( FLC ) . We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv . McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . [ Sen. 7, subscore: 1.00 ]: We report the cloning and characterization of the gamma ( gamma ) isoform of FCA from barley ( Hordeum vulgare cv . McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation . [ Sen. 8, subscore: 1.00 ]: McLeod ) . The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation . [ Sen. 9, subscore: 1.00 ]: The deduced protein contained two RNA recognition motifs ( RRMs ) , a glycine-rich region at the N-terminal end , a polyglutamine region immediately downstream of a WW domain . Barley FCA had greater protein sequence homology to wheat and rice FCA than to its Arabidopsis homolog . In developing barley embryos , FCA transcripts could be detected from 2 days after pollination ( DAP ) up to 40 DAP . FCA transcript levels in mature barley embryo were more abundant in non-germinated than in germinated seeds , with the levels declining as germination progressed . ABA inhibition of germination inhibited the decline of barley embryo FCA . Transient co-expression of FCA or a truncated FCA ( lacking RRM ) with maize VP1 promoter or wheat Em gene promoter in barley aleurone protoplasts led to increased VP1 and Em gene promoter activity . Barley FCA or truncated FCA localized in the nucleus suggested its role in gene regulation .
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Score: 8.00 | Title: Conservation and divergence of FCA function between Arabidopsis and rice .
| Author: Lee JH Cho YS Yoon HS Suh MC Moon J Lee I Weigel D Yun CH Kim JK .
| Journal: Plant Mol . Biol . Citation: V : 58 ( 6 ) P : 823-38 Year: 2005 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub16240176 Accession (PMID): 16240176 | Matching Sentences: [ Sen. 10, subscore: 2.00 ]: We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene . The beta and gamma transcripts of the OsFCA gene were detected via Northern analysis in the leaves , roots , and flowers of the plant . Flowers in younger stages exhibited higher transcript levels . These data suggest that intron3 may constitute a primary control point in the OsFCA pre-mRNA processing of rice . The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome . The constitutive expression of OsFCA resulted in no downregulation of FLC , but did result in the weak upregulation of SOC1 in the transgenic Arabidopsis . OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA . All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis . [ Sen. 12, subscore: 2.00 ]: Flowers in younger stages exhibited higher transcript levels . These data suggest that intron3 may constitute a primary control point in the OsFCA pre-mRNA processing of rice . The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome . The constitutive expression of OsFCA resulted in no downregulation of FLC , but did result in the weak upregulation of SOC1 in the transgenic Arabidopsis . OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA . All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis . [ Sen. 2, subscore: 1.00 ]: Although several genes have been identified in rice which are functionally equivalent to the flowering time genes in Arabidopsis , primarily genes involved in the photoperiod pathway , little data is available regarding the genes that function in the autonomous pathway in rice . In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA . The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA . However , the genetic structure of OsFCA is , in general , similar to that of FCA . RT-PCR and in silico analyses also showed that alternative splicing and polyadenylation at intron3 were conserved in the genetic expression of OsFCA . We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene . [ Sen. 3, subscore: 1.00 ]: Although several genes have been identified in rice which are functionally equivalent to the flowering time genes in Arabidopsis , primarily genes involved in the photoperiod pathway , little data is available regarding the genes that function in the autonomous pathway in rice . In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA . The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA . However , the genetic structure of OsFCA is , in general , similar to that of FCA . RT-PCR and in silico analyses also showed that alternative splicing and polyadenylation at intron3 were conserved in the genetic expression of OsFCA . We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene . The beta and gamma transcripts of the OsFCA gene were detected via Northern analysis in the leaves , roots , and flowers of the plant . [ Sen. 4, subscore: 1.00 ]: Although several genes have been identified in rice which are functionally equivalent to the flowering time genes in Arabidopsis , primarily genes involved in the photoperiod pathway , little data is available regarding the genes that function in the autonomous pathway in rice . In order to acquire further insight into the control of heading dates in rice , we isolated and conducted an expression analysis on OsFCA , which exhibited 38% sequence homology with Arabidopsis FCA . The N-terminal region of the OsFCA protein appears to be unusually rich in glycine-residues , unlike the N-terminal region found in FCA . However , the genetic structure of OsFCA is , in general , similar to that of FCA . RT-PCR and in silico analyses also showed that alternative splicing and polyadenylation at intron3 were conserved in the genetic expression of OsFCA . We were able to detect alpha , beta , and gamma transcripts , but not the delta transcript , of the OsFCA gene . The beta and gamma transcripts of the OsFCA gene were detected via Northern analysis in the leaves , roots , and flowers of the plant . Flowers in younger stages exhibited higher transcript levels . [ Sen. 13, subscore: 1.00 ]: These data suggest that intron3 may constitute a primary control point in the OsFCA pre-mRNA processing of rice . The overexpression of OsFCA cDNA , driven by the 35S promoter , was shown to partially rescue the late flowering phenotype of the fca mutant , suggesting that the functions of the OsFCA and the FCA are partially overlapped , despite the lack of an apparent FLC homologue in the rice genome . The constitutive expression of OsFCA resulted in no downregulation of FLC , but did result in the weak upregulation of SOC1 in the transgenic Arabidopsis . OsFCA overexpression did not result in a reduction of the gamma transcript levels of FCA in the transgenic Arabidopsis either , thereby suggesting that OsFCA had no effects on the autoregulation of Arabidopsis FCA . All of these results imply conservation and divergence in the functions of FCA between rice and Arabidopsis .
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Score: 4.00 | Title: OsFY , a homolog of AtFY , encodes a protein that can interact with OsFCA-gamma in rice ( Oryza sativa L ) .
| Author: Lu Q Xu ZK Song RT .
| Journal: Acta Biochim . Biophys . Sin . ( Shanghai ) Citation: V : 38 ( 7 ) P : 492-9 Year: 2006 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub16820865 Accession (PMID): 16820865 | Matching Sentences: [ Sen. 2, subscore: 2.00 ]: FCA and FY are flowering time related genes involved in the autonomous flowering pathway in Arabidopsis . FCA interacts with FY to regulate the alternative processing of FCA pre-mRNA . The FCA/FY interaction is also required for the regulation of FLC expression , a major floral repressor in Arabidopsis . However , it is not clear if the regulation of this autonomous flowering pathway is also present in monocot plants , such as rice . Recently , alternative RNA processing of OsFCA was observed in rice , which strongly suggested the existence of an autonomous flowering pathway in rice . In this work , we cloned the cDNA of the autonomous flowering pathway gene OsFY from rice . [ Sen. 1, subscore: 1.00 ]: FCA and FY are flowering time related genes involved in the autonomous flowering pathway in Arabidopsis . FCA interacts with FY to regulate the alternative processing of FCA pre-mRNA . The FCA/FY interaction is also required for the regulation of FLC expression , a major floral repressor in Arabidopsis . However , it is not clear if the regulation of this autonomous flowering pathway is also present in monocot plants , such as rice . Recently , alternative RNA processing of OsFCA was observed in rice , which strongly suggested the existence of an autonomous flowering pathway in rice . [ Sen. 10, subscore: 1.00 ]: In this work , we cloned the cDNA of the autonomous flowering pathway gene OsFY from rice . The predicted OsFY protein contained a conserved 7 WD-repeat region and at least two Pro-Pro-Leu-Pro motifs compared to Arabidopsis FY . The protein-protein interaction between OsFY and OsFCA-gamma , the key feature of their gene function , was also demonstrated using the yeast two-hybrid system . The GenBank database search provided evidence of expression for other autonomous pathway gene homologs in rice . These results indicate that the autonomous flowering pathway is present in monocots , and the regulation through FY and FCA interaction is conserved between monocots and dicots .
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Score: 4.00 | Title: Overexpression of the rFCA RNA recognition motif affects morphologies modifications in rice ( Oryza sativa L ) .
| Author: Hong F Attia K Wei C Li K He G Su W Zhang Q Qian X Yang J | Journal: Biosci Rep Citation: V : 27 P : 225-34 Year: 2007 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub17597396 Accession (PMID): 17597396 | Matching Sentences: [ Sen. 4, subscore: 2.00 ]: RNA recognition motifs as important regulators of gene expression are highly conserved in animals and plants . The FCA floral promotion gene in Arabidopsis encodes a protein , containing two RNA recognition motifs ( RRM ) and a WW protein interaction domain . Here we isolated FCA cDNA from rice . FCA in rice ( rFCA ) was homologous to FCA-gamma of Arabidopsis and contained conserved domains . To investigate the function of RRM domain , fragment RRM1 and RRM2 of rFCA were introduced into rice subspecies Oryza sativa L subsp . Indica var . 9311 and another rice subspecies Oryza sativa L subsp . Japonica var . zhonghua11 transformation . [ Sen. 2, subscore: 1.00 ]: RNA recognition motifs as important regulators of gene expression are highly conserved in animals and plants . The FCA floral promotion gene in Arabidopsis encodes a protein , containing two RNA recognition motifs ( RRM ) and a WW protein interaction domain . Here we isolated FCA cDNA from rice . FCA in rice ( rFCA ) was homologous to FCA-gamma of Arabidopsis and contained conserved domains . To investigate the function of RRM domain , fragment RRM1 and RRM2 of rFCA were introduced into rice subspecies Oryza sativa L subsp . Indica var . [ Sen. 3, subscore: 1.00 ]: RNA recognition motifs as important regulators of gene expression are highly conserved in animals and plants . The FCA floral promotion gene in Arabidopsis encodes a protein , containing two RNA recognition motifs ( RRM ) and a WW protein interaction domain . Here we isolated FCA cDNA from rice . FCA in rice ( rFCA ) was homologous to FCA-gamma of Arabidopsis and contained conserved domains . To investigate the function of RRM domain , fragment RRM1 and RRM2 of rFCA were introduced into rice subspecies Oryza sativa L subsp . Indica var . 9311 and another rice subspecies Oryza sativa L subsp .
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Score: 4.00 | Title: Abscisic acid does not disrupt either the Arabidopsis FCA-FY interaction or its rice counterpart in vitro .
| Author: Jang YH Lee JH Kim JK | Journal: Plant Cell Physiol Citation: V : 49 P : 1898-901 Year: 2008 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub18854333 Accession (PMID): 18854333 | Matching Sentences: [ Sen. 1, subscore: 1.00 ]: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY . From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment . This result prompted us to examine the effect of ABA on the FCA-FY interaction . In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY . Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered . [ Sen. 3, subscore: 1.00 ]: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY . From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment . This result prompted us to examine the effect of ABA on the FCA-FY interaction . In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY . Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered . [ Sen. 4, subscore: 1.00 ]: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY . From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment . This result prompted us to examine the effect of ABA on the FCA-FY interaction . In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY . Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered . [ Sen. 5, subscore: 1.00 ]: We examined the effect of ( + ) -ABA on the in vitro interaction of rice FCA and FY homologs , OsFCA and OsFY . From this analysis , we found no disruption of the OsFCA-OsFY complexes by ABA treatment . This result prompted us to examine the effect of ABA on the FCA-FY interaction . In these experiments , we could not reproduce the inhibitory effect of ( + ) -ABA on the interaction between FCA and FY . Based on these combined results , we believe that the inhibitory effect of ( + ) -ABA on the FCA-FY interaction should be cautiously reconsidered .
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