| 136 matches found in 59 documents. Search time: 0.005 seconds. |
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Score: 13.00 |
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| Title: Light-regulated and cell-specific expression of tomato rbcS-gusA and rice rbcS-gusA fusion genes in transgenic rice .
| | Author: Kyozuka J McElroy D Hayakawa T Xie Y Wu R Shimamoto K | | Journal: Plant Physiol .
Citation: V : 102 ( 3 ) P : 991-1000 Year: 1993 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8278540 Accession (PMID): 8278540 | | Abstract: A previously isolated rice ( Oryza sativa ) rbcS gene was further characterized .
This analysis revealed specific sequences in the 5 regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species .
In transgenic rice plants , we examined the expression of the beta-glucuronidase ( gusA ) reporter gene directed by the 2 . 8-kb promoter region of the rice rbcS gene .
To examine differences in the regulation of monocotyledonous and dicotyledonous rbcS promoters , the activity of a tomato rbcS promoter was also investigated in transgenic rice plants .
Our results indicated that both rice and tomato rbcS promoters confer mesophyll-specific expression of the gusA reporter gene in transgenic rice plants and that this expression is induced by light .
However , the expression level of the rice rbcS-gusA gene was higher than that of the tomato rbcS-gusA gene , suggesting the presence of quantitative differences in the activity of these particular monocotyledonous and dicotyledonous rbcS promoters in transgenic rice .
Histochemical analysis of rbcS-gusA gene expression showed that the observed light induction was only found in mesophyll cells .
Furthermore , it was demonstrated that the light regulation of rice rbcS-gusA gene expression was primarily at the level of mRNA accumulation .
We show that the rice rbcS gene promoter should be useful for expression of agronomically important genes for genetic engineering of monocotyledonous species . | Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: However , the expression level of the rice rbcS-gusA gene was higher than that of the tomato rbcS-gusA gene , suggesting the presence of quantitative differences in the activity of these particular monocotyledonous and dicotyledonous rbcS promoters in transgenic rice .
[ Sen. 2, subscore: 2.00 ]: This analysis revealed specific sequences in the 5 regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species .
[ Sen. 4, subscore: 2.00 ]: To examine differences in the regulation of monocotyledonous and dicotyledonous rbcS promoters , the activity of a tomato rbcS promoter was also investigated in transgenic rice plants .
[ Sen. 1, subscore: 1.00 ]: A previously isolated rice ( Oryza sativa ) rbcS gene was further characterized .
[ Sen. 3, subscore: 1.00 ]: In transgenic rice plants , we examined the expression of the beta-glucuronidase ( gusA ) reporter gene directed by the 2 . 8-kb promoter region of the rice rbcS gene .
[ Sen. 5, subscore: 1.00 ]: Our results indicated that both rice and tomato rbcS promoters confer mesophyll-specific expression of the gusA reporter gene in transgenic rice plants and that this expression is induced by light .
[ Sen. 7, subscore: 1.00 ]: Histochemical analysis of rbcS-gusA gene expression showed that the observed light induction was only found in mesophyll cells .
[ Sen. 8, subscore: 1.00 ]: Furthermore , it was demonstrated that the light regulation of rice rbcS-gusA gene expression was primarily at the level of mRNA accumulation .
[ Sen. 9, subscore: 1.00 ]: We show that the rice rbcS gene promoter should be useful for expression of agronomically important genes for genetic engineering of monocotyledonous species .
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Score: 10.00 |
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| Title: Availability of Rubisco Small Subunit Up-Regulates the Transcript Levels of Large Subunit for Stoichiometric Assembly of Its Holoenzyme in Rice .
| | Author: Suzuki Y Makino A | | Journal: Plant Physiol Citation: V : P : Year: 2012 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub22811433 Accession (PMID): 22811433 | | Abstract: Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome .
For synthesis of the Rubisco holoenzyme , both genes need to be expressed coordinately .
To investigate this molecular mechanism , protein synthesis of two subunits of Rubisco was characterized in transgenic rice ( Oryza sativa L ) plants with overexpression or antisense suppression of RBCS gene .
Total RBCS and rbcL mRNA levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants , although an increase in total RBCS mRNA level was greater .
In RBCS-antisense plants , the levels of these mRNAs and synthesis of the corresponding proteins declined to a similar extent .
The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes .
The level of rbcL mRNA in turn was tightly correlated with the amount of RbcL synthesized , the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype .
Polysome loading of rbcL mRNA was not changed .
These results demonstrate that the availability of RBCS protein up-regulates gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme .
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[ Sen. 4, subscore: 4.00 ]: Total RBCS and rbcL mRNA levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants , although an increase in total RBCS mRNA level was greater .
[ Sen. 1, subscore: 1.00 ]: Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome .
[ Sen. 3, subscore: 1.00 ]: To investigate this molecular mechanism , protein synthesis of two subunits of Rubisco was characterized in transgenic rice ( Oryza sativa L ) plants with overexpression or antisense suppression of RBCS gene .
[ Sen. 5, subscore: 1.00 ]: In RBCS-antisense plants , the levels of these mRNAs and synthesis of the corresponding proteins declined to a similar extent .
[ Sen. 6, subscore: 1.00 ]: The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes .
[ Sen. 7, subscore: 1.00 ]: The level of rbcL mRNA in turn was tightly correlated with the amount of RbcL synthesized , the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype .
[ Sen. 9, subscore: 1.00 ]: These results demonstrate that the availability of RBCS protein up-regulates gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme .
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Score: 8.00 |
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| Title: Developmental characteristics and aluminum resistance of root border cells in rice seedlings .
| | Author: Cai M Zhang S Xing C Wang F Ning W Lei Z | | Journal: Plant Sci Citation: V : 180 P : 702-8 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21421421 Accession (PMID): 21421421 | | Abstract: The developmental characteristics of root border cells ( RBCs ) and their role in protection of root apices of rice seedling from Al toxicity were evaluated in two rice ( Oryza sativa L ) cultivars differing in Al tolerance .
Root elongation and RBCs viability were used as indicators for Al effects .
The formation of RBCs and the emergence of the root tip occurred almost simultaneously .
Treatment of the root with Al inhibited root elongation and increased Al accumulation in the root tips .
Physical removal of RBCs from root tips resulted in a more severe inhibition of root elongation and a higher Al accumulation in the root tips .
These effects were more pronounced in the Al-sensitive rice cultivar ( II You 6216 ) than that in the Al-tolerant rice cultivar ( II You 838 ) .
The relative viability of attached and detached RBCs decreased with increasing Al concentrations .
Al also induced a thicker mucilage layer surrounding attached RBCs of both cultivars , and detached RBCs did not .
Maintaining the abundant live RBCs encapsulated root tip and enhancing their mucilage secretion , appear to be important in alleviating Al toxicity and in allowing exclusion of Al from the rice root apex | Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Al also induced a thicker mucilage layer surrounding attached RBCs of both cultivars , and detached RBCs did not .
[ Sen. 1, subscore: 1.00 ]: The developmental characteristics of root border cells ( RBCs ) and their role in protection of root apices of rice seedling from Al toxicity were evaluated in two rice ( Oryza sativa L ) cultivars differing in Al tolerance .
[ Sen. 2, subscore: 1.00 ]: Root elongation and RBCs viability were used as indicators for Al effects .
[ Sen. 3, subscore: 1.00 ]: The formation of RBCs and the emergence of the root tip occurred almost simultaneously .
[ Sen. 5, subscore: 1.00 ]: Physical removal of RBCs from root tips resulted in a more severe inhibition of root elongation and a higher Al accumulation in the root tips .
[ Sen. 7, subscore: 1.00 ]: The relative viability of attached and detached RBCs decreased with increasing Al concentrations .
[ Sen. 9, subscore: 1.00 ]: Maintaining the abundant live RBCs encapsulated root tip and enhancing their mucilage secretion , appear to be important in alleviating Al toxicity and in allowing exclusion of Al from the rice root apex
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Score: 8.00 |
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| Title: Effect of individual suppression of RBCS multigene family on Rubisco contents in rice leaves .
| | Author: Ogawa S Suzuki Y Yoshizawa R Kanno K Makino A | | Journal: Plant Cell Environ Citation: V : 35 P : 546-53 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21951138 Accession (PMID): 21951138 | | Abstract: In higher plants , a small subunit of Rubisco is encoded for by an RBCS multigene family in the nuclear genome .
However , it is unknown how each multigene member contributes to the accumulation of Rubisco holoenzyme .
Here , four RBCS genes that are highly expressed in leaf blaedes of rice ( Oryza sativa L ) were individually suppressed by RNAi , and the effects on leaf Rubisco content were examined at seedling , vegetative and reproductive stages .
Rubisco contents in each transgenic line declined irrespective of growth stage , and the ratios of Rubisco-N to total N were 66-96% of wild-type levels .
The mRNA levels of the suppressed RBCS genes declined significantly , whereas those of the unsuppressed ones did not change drastically .
These results indicate that four RBCS genes all contribute to accumulation of Rubisco holoenzyme irrespective of growth stage and that suppression of one RBCS gene is not fully compensated by other RBCS genes .
Additionally , the mRNA levels of the large subunit of Rubisco showed a change similar to that of total RBCS mRNA level irrespective of genotype and growth stage .
These results suggest that gene expression of RBCS and RBCL is regulated in a coordinated manner at the transcript level in rice .
| Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: These results indicate that four RBCS genes all contribute to accumulation of Rubisco holoenzyme irrespective of growth stage and that suppression of one RBCS gene is not fully compensated by other RBCS genes .
[ Sen. 1, subscore: 1.00 ]: In higher plants , a small subunit of Rubisco is encoded for by an RBCS multigene family in the nuclear genome .
[ Sen. 3, subscore: 1.00 ]: Here , four RBCS genes that are highly expressed in leaf blaedes of rice ( Oryza sativa L ) were individually suppressed by RNAi , and the effects on leaf Rubisco content were examined at seedling , vegetative and reproductive stages .
[ Sen. 5, subscore: 1.00 ]: The mRNA levels of the suppressed RBCS genes declined significantly , whereas those of the unsuppressed ones did not change drastically .
[ Sen. 7, subscore: 1.00 ]: Additionally , the mRNA levels of the large subunit of Rubisco showed a change similar to that of total RBCS mRNA level irrespective of genotype and growth stage .
[ Sen. 8, subscore: 1.00 ]: These results suggest that gene expression of RBCS and RBCL is regulated in a coordinated manner at the transcript level in rice .
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Score: 7.00 |
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| Title: The promoter of rbcS in a C3 plant ( rice ) directs organ-specific , light-dependent expression in a C4 plant ( maize ) , but does not confer bundle sheath cell-specific expression .
| | Author: Nomura M Katayama K Nishimura A Ishida Y Ohta S Komari T Miyao-Tokutomi M Tajima S Matsuoka M | | Journal: Plant Mol . Biol . Citation: V : 44 ( 1 ) P : 99-106 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11094984 Accession (PMID): 11094984 | | Abstract: The small subunit of ribulose-bisphosphate carboxylase ( Rubisco ) , encoded by rbcS , is essential for photosynthesis in both C3 and C4 plants , even though the cell specificity of rbcS expression is different between C3 and C4 plants .
The C3 rbcS is specifically expressed in mesophyll cells , while the C4 rbcS is expressed in bundle sheath cells , and not mesophyll cells .
Two chimeric genes were constructed consisting of the structural gene encoding beta-glucuronidase ( GUS ) controlled by the two promoters from maize ( C4 ) and rice ( C3 ) rbcS genes .
These constructs were introduced into a C4 plant , maize .
Both chimeric genes were specifically expressed in photosynthetic organs , such as leaf blade , but not in non-photosynthetic organs .
The expressions of the genes were also regulated by light .
However , the rice promoter drove the GUS activity mainly in mesophyll cells and relatively low in bundle sheath cells , while the maize rbcS promoter induced the activity specifically in bundle sheath cells .
These results suggest that the rice promoter contains some cis-acting elements responding in an organ-specific and light-inducible regulation manner in maize but does not contain element ( s ) for bundle sheath cell-specific expression , while the maize promoter does contain such element ( s ) .
Based on this result , we discuss the similarities and differences between the rice ( C3 ) and maize ( C4 ) rbcS promoter in terms of the evolution of the C4 photosynthetic gene . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The small subunit of ribulose-bisphosphate carboxylase ( Rubisco ) , encoded by rbcS , is essential for photosynthesis in both C3 and C4 plants , even though the cell specificity of rbcS expression is different between C3 and C4 plants .
[ Sen. 2, subscore: 2.00 ]: The C3 rbcS is specifically expressed in mesophyll cells , while the C4 rbcS is expressed in bundle sheath cells , and not mesophyll cells .
[ Sen. 3, subscore: 1.00 ]: Two chimeric genes were constructed consisting of the structural gene encoding beta-glucuronidase ( GUS ) controlled by the two promoters from maize ( C4 ) and rice ( C3 ) rbcS genes .
[ Sen. 7, subscore: 1.00 ]: However , the rice promoter drove the GUS activity mainly in mesophyll cells and relatively low in bundle sheath cells , while the maize rbcS promoter induced the activity specifically in bundle sheath cells .
[ Sen. 9, subscore: 1.00 ]: Based on this result , we discuss the similarities and differences between the rice ( C3 ) and maize ( C4 ) rbcS promoter in terms of the evolution of the C4 photosynthetic gene .
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Score: 7.00 |
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| Title: Isolation and characterization of a green it issue-specific promoter from pigeonpea [ Cajanus cajan ( L ) Millsp . ] .
| | Author: Panguluri SK Sridhar J Jagadish B Sharma PC Kumar PA .
| | Journal: Indian J Exp . Biol . Citation: V : 43 ( 4 ) P : 369-72 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15875723 Accession (PMID): 15875723 | | Abstract: Expression of rbcS genes encoding small subunit of rubisco , most abundant protein in green it issue , is regulated by at least three parameters--tissue type , light conditions and stage of development .
One of the green it issue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR .
Expression of uidA gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter , clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier .
Study of the sequence similarity with pea rbcS3A promoter , especially the region ( boxes I and III ) that is required for rbcS3A expression , showed more than 50% divergence .
In contrast , pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters .
| Matching Sentences:
[ Sen. 3, subscore: 2.00 ]: Expression of uidA gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter , clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier .
[ Sen. 4, subscore: 2.00 ]: Study of the sequence similarity with pea rbcS3A promoter , especially the region ( boxes I and III ) that is required for rbcS3A expression , showed more than 50% divergence .
[ Sen. 1, subscore: 1.00 ]: Expression of rbcS genes encoding small subunit of rubisco , most abundant protein in green it issue , is regulated by at least three parameters--tissue type , light conditions and stage of development .
[ Sen. 2, subscore: 1.00 ]: One of the green it issue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR .
[ Sen. 5, subscore: 1.00 ]: In contrast , pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters .
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Score: 7.00 |
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| Title: Increased Rubisco Content in Transgenic Rice Transformed with the Sense rbcS Gene .
| | Author: Suzuki Y Ohkubo M Hatakeyama H Ohashi K Yoshizawa R Kojima S Hayakawa T Yamaya T Mae T Makino A | | Journal: Plant Cell Physiol .
Citation: V : 48 ( 4 ) P : 626-37 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17379698 Accession (PMID): 17379698 | | Abstract: Rice ( Oryza sativa L ) plants with substantially increased Rubisco content were obtained by Agrobacterium-mediated transformation with the rice rbcS sense gene under the control of the rice rbcS promoter .
The primary transformants were screened for the ratio of Rubisco to leaf-N content , and the transformants with >120% wild-type levels of Rubisco were selected .
In the progeny of the selected lines of the transformants , the mRNA levels of one member of the rbcS gene family were increased from 3 . 9 to 6 . 2-fold , whereas those of other members of the rbcS gene family were unchanged .
The total levels of rbcS mRNA were increased from 2 . 1 to 2 . 8-fold .
The levels of rbcL mRNA were increased from 1 . 2 to 1 . 9-fold .
Rubisco protein content was significantly increased by 30% on a leaf area basis .
The ratio of Rubisco-N to leaf-N was also increased by 10-20% , irrespective of N treatment .
The specific activity of Rubisco per unit of enzyme protein was not different .
However , light-saturated photosynthesis was not enhanced even when the rate was measured at low [ CO ( 2 ) ] where Rubisco becomes limiting for photosynthesis .
Some lines showed lower photosynthesis at high [ CO ( 2 ) ] ( >60 Pa ) .
We conclude that introduction of additional sense rbcS leads to overexpression of rbcS and that this overexpression slightly up-regulates the gene expression of rbcL at the transcript level and enhances the amount of Rubisco holoenzyme .
However , overproduction of Rubisco protein does not improve photosynthesis .
| Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: Rice ( Oryza sativa L ) plants with substantially increased Rubisco content were obtained by Agrobacterium-mediated transformation with the rice rbcS sense gene under the control of the rice rbcS promoter .
[ Sen. 3, subscore: 2.00 ]: In the progeny of the selected lines of the transformants , the mRNA levels of one member of the rbcS gene family were increased from 3 . 9 to 6 . 2-fold , whereas those of other members of the rbcS gene family were unchanged .
[ Sen. 11, subscore: 2.00 ]: We conclude that introduction of additional sense rbcS leads to overexpression of rbcS and that this overexpression slightly up-regulates the gene expression of rbcL at the transcript level and enhances the amount of Rubisco holoenzyme .
[ Sen. 4, subscore: 1.00 ]: The total levels of rbcS mRNA were increased from 2 . 1 to 2 . 8-fold .
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Score: 6.00 |
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| Title: [ Specific expression of the foreign gene regulated by the rice rbcS promoter in transgenic rice ] | | Author: Liu QQ Yu HX Zhang WJ Wang HM Gu MH .
| | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 31 ( 3 ) P : 247-53 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15961898 Accession (PMID): 15961898 | | Abstract: To use different types of promoters in transgenic rice research , the 5-upstream regulation region of rice Rubisco small subunit gene ( rbcS ) was cloned from a Chinese cultivar Wuyunjing 8 , and its sequences were confirmed by comparison with the known genome sequences of both japonica and indica rice .
The cloned rbcS promoter was fused to the 5-upstream of GUS ( beta-glucuronidase ) coding region in a binary vector , and introduced into rice by Agrogacterium-mediated transformation .
The integration of the rbcS-GUS fusion gene in transgenic rice was confirmed by PCR analysis .
The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in leaf blade and sheath than in other organs of transgenic rice plants , and the GUS activity was restricted to the mesophyll cells of leaf it issue , which showed that the rice rbcS promoter could control not only the it issue but also the cell-specific expression of foreign genes in transgenic rice .
The present results also demonstrated that light induction had a significant effect on the enhancement of transgenes expression when regulated by the rice rbcS promoter in transgenic rice .
Our results showed that the rice rbcS promoter might be very useful for the expression of target genes in transgenic rice , with particularly high efficiency in leaf it issues .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To use different types of promoters in transgenic rice research , the 5-upstream regulation region of rice Rubisco small subunit gene ( rbcS ) was cloned from a Chinese cultivar Wuyunjing 8 , and its sequences were confirmed by comparison with the known genome sequences of both japonica and indica rice .
[ Sen. 2, subscore: 1.00 ]: The cloned rbcS promoter was fused to the 5-upstream of GUS ( beta-glucuronidase ) coding region in a binary vector , and introduced into rice by Agrogacterium-mediated transformation .
[ Sen. 3, subscore: 1.00 ]: The integration of the rbcS-GUS fusion gene in transgenic rice was confirmed by PCR analysis .
[ Sen. 4, subscore: 1.00 ]: The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in leaf blade and sheath than in other organs of transgenic rice plants , and the GUS activity was restricted to the mesophyll cells of leaf it issue , which showed that the rice rbcS promoter could control not only the it issue but also the cell-specific expression of foreign genes in transgenic rice .
[ Sen. 5, subscore: 1.00 ]: The present results also demonstrated that light induction had a significant effect on the enhancement of transgenes expression when regulated by the rice rbcS promoter in transgenic rice .
[ Sen. 6, subscore: 1.00 ]: Our results showed that the rice rbcS promoter might be very useful for the expression of target genes in transgenic rice , with particularly high efficiency in leaf it issues .
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Score: 6.00 |
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| Title: Functional incorporation of sorghum small subunit increases the catalytic turnover rate of Rubisco in transgenic rice .
| | Author: Ishikawa C Hatanaka T Misoo S Miyake C Fukayama H | | Journal: Plant Physiol Citation: V : 156 P : 1603-11 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21562335 Accession (PMID): 21562335 | | Abstract: Rubisco limits photosynthetic CO ( 2 ) fixation because of its low catalytic turnover rate ( k ( cat ) ) and competing oxygenase reaction .
Previous attempts to improve the catalytic efficiency of Rubisco by genetic engineering have gained little progress .
Here we demonstrate that the introduction of the small subunit ( RbcS ) of high k ( cat ) Rubisco from the C ( 4 ) plant sorghum ( Sorghum bicolor ) significantly enhances k ( cat ) of Rubisco in transgenic rice ( Oryza sativa ) .
Three independent transgenic lines expressed sorghum RbcS at a high level , accounting for 30% , 44% , and 79% of the total RbcS .
Rubisco was likely present as a chimera of sorghum and rice RbcS , and showed 1 . 32 to 1 . 50-fold higher k ( cat ) than in nontransgenic rice .
Rubisco from transgenic lines showed a higher K ( m ) for CO ( 2 ) and slightly lower specificity for CO ( 2 ) than nontransgenic controls .
These results suggest that Rubisco in rice transformed with sorghum RbcS partially acquires the catalytic properties of sorghum Rubisco Rubisco content in transgenic lines was significantly increased over wild-type levels but Rubisco activation was slightly decreased .
The expression of sorghum RbcS did not affect CO ( 2 ) assimilation rates under a range of CO ( 2 ) partial pressures .
The J ( max ) /V ( cmax ) ratio was significantly lower in transgenic line compared to the nontransgenic plants .
These observations suggest that the capacity of electron transport is not sufficient to support the increased Rubisco capacity in transgenic rice .
Although the photosynthetic rate was not enhanced , the strategy presented here opens the way to engineering Rubisco for improvement of photosynthesis and productivity in the future .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Three independent transgenic lines expressed sorghum RbcS at a high level , accounting for 30% , 44% , and 79% of the total RbcS .
[ Sen. 3, subscore: 1.00 ]: Here we demonstrate that the introduction of the small subunit ( RbcS ) of high k ( cat ) Rubisco from the C ( 4 ) plant sorghum ( Sorghum bicolor ) significantly enhances k ( cat ) of Rubisco in transgenic rice ( Oryza sativa ) .
[ Sen. 5, subscore: 1.00 ]: Rubisco was likely present as a chimera of sorghum and rice RbcS , and showed 1 . 32 to 1 . 50-fold higher k ( cat ) than in nontransgenic rice .
[ Sen. 7, subscore: 1.00 ]: These results suggest that Rubisco in rice transformed with sorghum RbcS partially acquires the catalytic properties of sorghum Rubisco Rubisco content in transgenic lines was significantly increased over wild-type levels but Rubisco activation was slightly decreased .
[ Sen. 8, subscore: 1.00 ]: The expression of sorghum RbcS did not affect CO ( 2 ) assimilation rates under a range of CO ( 2 ) partial pressures .
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Score: 5.00 |
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| Title: [ Expression of the GUS fusion gene controlled by the tomato rbcS3A promoter in transgenic rice ] | | Author: Liu QQ Yu HX Zhang WJ Gong ZY Gu MH | | Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 33 P : 251-7 Year: 2007 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub17556813 Accession (PMID): 17556813 | | Abstract: To use different types of promoters in transgenic rice research , the 1 . 1 kb 5-upstream regulation region of one of the tomato ( Solanum tuberosum L ) Rubisco small subunit gene , rbcS3A , was cloned and its sequences were confirmed by comparison with the known sequences in GenBank .
The cloned rbcS3A promoter was fused to the 5-upstream of GUS ( beta-glucuronidase ) coding region in a binary vector , and introduced into an elite japonica rice variety by Agrogacterium-mediated transformation .
The integration of the GUS fusion gene into the genome of transgenic rice was confirmed by both PCR and Southern blot analysis .
The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in stem , leaf blade and sheath than in other organs of transgenic rice plants , and showed highest in the stem , which implies that the tomato rbcS3A promoter can make it issue-specific , in particular in the stem , expression of foreign genes in transgenic rice .
The results present here also demonstrate that light induction had no effect on the expression of the foreign gene when regulated by the tomato rbcS3A promoter in transgenic rice .
Our results show that the cloned tomato rbcS3A promoter might be very useful for the expression of target genes in transgenic rice , with particularly high efficiency in stem it issues .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: To use different types of promoters in transgenic rice research , the 1 . 1 kb 5-upstream regulation region of one of the tomato ( Solanum tuberosum L ) Rubisco small subunit gene , rbcS3A , was cloned and its sequences were confirmed by comparison with the known sequences in GenBank .
[ Sen. 2, subscore: 1.00 ]: The cloned rbcS3A promoter was fused to the 5-upstream of GUS ( beta-glucuronidase ) coding region in a binary vector , and introduced into an elite japonica rice variety by Agrogacterium-mediated transformation .
[ Sen. 4, subscore: 1.00 ]: The results of both histochemical staining and quantitative analysis of GUS activity showed that the expression level of GUS fusion gene was significantly stronger in stem , leaf blade and sheath than in other organs of transgenic rice plants , and showed highest in the stem , which implies that the tomato rbcS3A promoter can make it issue-specific , in particular in the stem , expression of foreign genes in transgenic rice .
[ Sen. 5, subscore: 1.00 ]: The results present here also demonstrate that light induction had no effect on the expression of the foreign gene when regulated by the tomato rbcS3A promoter in transgenic rice .
[ Sen. 6, subscore: 1.00 ]: Our results show that the cloned tomato rbcS3A promoter might be very useful for the expression of target genes in transgenic rice , with particularly high efficiency in stem it issues .
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