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Score: 15.00 |
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| Title: Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 ( Xa5 ) in comparison with its homolog TFIIAgamma1 .
| | Author: Jiang GH Xia ZH Zhou YL Wan J Li DY Chen RS Zhai WX Zhu LH .
| | Journal: Citation: V : ( ) P : 1-13 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16444514 Accession (PMID): 16444514 | | Abstract: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
The rice has an addition gene for TFIIAgamma exists on chromosome 1 ( TFIIAgamma1 ) .
Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
However , no expression of TFIIAgamma1 could be detected in the panicle by reverse transcriptase-polymerase chain reaction .
To compare the structural difference between the Xa5/xa5 and TFIIAgamma1 proteins , 3-D structures were predicted using computer-aided modeling techniques .
The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
| Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
[ Sen. 3, subscore: 3.00 ]: Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
[ Sen. 8, subscore: 2.00 ]: The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
[ Sen. 10, subscore: 2.00 ]: The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
[ Sen. 1, subscore: 1.00 ]: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
[ Sen. 5, subscore: 1.00 ]: Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
[ Sen. 9, subscore: 1.00 ]: The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
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Score: 15.00 |
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| Title: Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 ( Xa5 ) in comparison with its homolog TFIIAgamma1 .
| | Author: Jiang GH Xia ZH Zhou YL Wan J Li DY Chen RS Zhai WX Zhu LH .
| | Journal: Mol . Genet . Genomics Citation: V : 275 ( 4 ) P : 354-66 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16614777 Accession (PMID): 16614777 | | Abstract: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
The rice has an addition gene for TFIIAgamma exists on chromosome 1 ( TFIIAgamma1 ) .
Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
However , no expression of TFIIAgamma1 could be detected in the panicle by reverse transcriptase-polymerase chain reaction .
To compare the structural difference between the Xa5/xa5 and TFIIAgamma1 proteins , 3-D structures were predicted using computer-aided modeling techniques .
The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively . | Matching Sentences:
[ Sen. 2, subscore: 5.00 ]: However , xa5 has not been demonstrated by a complementation test In this study , we introduced the dominant allele Xa5 into a homozygous xa5-line , which was developed from a cross between IRBB5 ( an indica variety with xa5 ) and Nipponbare ( a japonica variety with Xa5 ) .
[ Sen. 3, subscore: 3.00 ]: Transformation of Xa5 and subsequent segregation analysis confirmed that xa5 is a V39E substitution variant of the gene for TFIIAgamma on chromosome 5 ( TFIIAgamma5 or Xa5 ) .
[ Sen. 8, subscore: 2.00 ]: The modeled structures of Xa5 ( xa5 ) and TFIIAgamma1 fit well with the structure of TFIIA small subunit from human , suggesting that they may all act as a small subunit of TFIIA .
[ Sen. 10, subscore: 2.00 ]: The structural analysis indicates that xa5 and Xa5 potentially retain their basic transcription factor function , which , in turn , may mediate the novel pathway for bacterial blight resistance and susceptibility , respectively .
[ Sen. 1, subscore: 1.00 ]: The recessive gene xa5 for resistance to bacterial blight resistance of rice is located on chromosome 5 , and evidence based on genetic recombination has been shown to encode a small subunit of the basal transcription factor IIA ( Iyer and McCouch in MPMI 17 ( 12 ) : 1348-1354 , 2004 ) .
[ Sen. 5, subscore: 1.00 ]: Analysis of the expression patterns of Xa5 ( TFIIAgamma5 ) /xa5 and TFIIAgamma1 revealed that both the genes are constitutively expressed in different rice organs .
[ Sen. 9, subscore: 1.00 ]: The E39V substitution in the xa5 protein occurs in the alpha-helix domain , a supposed conservative substitutable site , which should not affect the basal transcription function of TFIIAgamma .
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Score: 10.00 |
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| Title: Transcription activator-like type III effector AvrXa27 depends on OsTFIIAgamma5 for the activation of Xa27 transcription in rice that triggers disease resistance to Xanthomonas oryzae pv . oryzae .
| | Author: Gu K Tian D Qiu C Yin Z | | Journal: Mol Plant Pathol Citation: V : 10 P : 829-35 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19849788 Accession (PMID): 19849788 | | Abstract: The transcription activator-like ( TAL ) type III effector AvrXa27 from Xanthomonas oryzae pv . oryzae ( Xoo ) strain PXO99 ( A ) activates the transcription of the host resistance gene Xa27 , which results in disease resistance to bacterial blight ( BB ) in rice .
In this study , we show that AvrXa27-activated Xa27 transcription requires host general transcription factor OsTFIIAgamma5 .
The V39E substitution in OsTFIIAgamma5 , encoded by the recessive resistance gene xa5 in rice , greatly attenuates this activation in xa5 and Xa27 double homozygotes on inoculation with Xa27-incompatible strains .
The xa5 gene also causes attenuation in the induction of Xa27 by AvrXa27 expressed in rice .
The xa5-mediated attenuation of Xa27-mediated resistance to PXO99 ( A ) is recessive .
Intriguingly , xa5-mediated resistance to xa5-incompatible strains is also down-regulated in the xa5 and Xa27 double homozygotes .
In addition , AvrXa27 expressed in planta shows weak virulence activity in the xa5 genetic background and causes enhanced susceptibility of the plants to BB inoculation .
The results suggest that TAL effectors target host general transcription factors to directly manipulate the host transcriptional machinery for virulence and/or avirulence .
The identification of xa5-mediated attenuation of Xa27-mediated resistance to Xoo provides a guideline for breeding resistance to BB when pyramiding xa5 with other resistance genes .
| Matching Sentences:
[ Sen. 6, subscore: 3.00 ]: Intriguingly , xa5-mediated resistance to xa5-incompatible strains is also down-regulated in the xa5 and Xa27 double homozygotes .
[ Sen. 3, subscore: 2.00 ]: The V39E substitution in OsTFIIAgamma5 , encoded by the recessive resistance gene xa5 in rice , greatly attenuates this activation in xa5 and Xa27 double homozygotes on inoculation with Xa27-incompatible strains .
[ Sen. 9, subscore: 2.00 ]: The identification of xa5-mediated attenuation of Xa27-mediated resistance to Xoo provides a guideline for breeding resistance to BB when pyramiding xa5 with other resistance genes .
[ Sen. 4, subscore: 1.00 ]: The xa5 gene also causes attenuation in the induction of Xa27 by AvrXa27 expressed in rice .
[ Sen. 5, subscore: 1.00 ]: The xa5-mediated attenuation of Xa27-mediated resistance to PXO99 ( A ) is recessive .
[ Sen. 7, subscore: 1.00 ]: In addition , AvrXa27 expressed in planta shows weak virulence activity in the xa5 genetic background and causes enhanced susceptibility of the plants to BB inoculation .
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Score: 6.00 |
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| Title: Genetic and functional characterization of the rice bacterial blight disease resistance gene xa5 .
| | Author: Iyer-Pascuzzi AS Jiang H Huang L McCouch SR | | Journal: Phytopathology Citation: V : 98 P : 289-95 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18944079 Accession (PMID): 18944079 | | Abstract: Xanthomonas oryzae pv . oryzae is the causal agent of rice bacterial blight , a destructive rice disease worldwide .
The gene xa5 provides race-specific resistance to X oryzae pv . oryzae , and encodes the small subunit of transcription factor IIA .
How xa5 functions in bacterial blight resistance is not well understood , and its recessive gene action is disputed .
Here we show that xa5 is inherited in a completely recessive manner and the susceptible allele Xa5 is fully dominant .
In accordance with this , bacterial growth in heterozygous and homozygous susceptible lines is not significantly different .
Further , one allele of Xa5 is sufficient to promote disease in previously resistant plants ; additional copies are not predictive of increased lesion length .
Surprisingly , a resistant nearly isogenic line ( NIL ) of an indica variety sustains high levels of bacterial populations compared to the susceptible NIL , yet the resistant plants restrict symptom expression .
In contrast , in japonica NILs , bacterial population dynamics differ in resistant and susceptible genotypes .
However , both resistant indica and japonica plants delay bacterial movement down the leaf .
These results support a model in which xa5-mediated recessive resistance is the result of restricted bacterial movement , but not restricted multiplication .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Here we show that xa5 is inherited in a completely recessive manner and the susceptible allele Xa5 is fully dominant .
[ Sen. 2, subscore: 1.00 ]: The gene xa5 provides race-specific resistance to X oryzae pv . oryzae , and encodes the small subunit of transcription factor IIA .
[ Sen. 3, subscore: 1.00 ]: How xa5 functions in bacterial blight resistance is not well understood , and its recessive gene action is disputed .
[ Sen. 6, subscore: 1.00 ]: Further , one allele of Xa5 is sufficient to promote disease in previously resistant plants ; additional copies are not predictive of increased lesion length .
[ Sen. 10, subscore: 1.00 ]: These results support a model in which xa5-mediated recessive resistance is the result of restricted bacterial movement , but not restricted multiplication .
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Score: 5.00 |
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| Title: Inheritance of bacterial blight resistance in the rice cultivar Ajaya and high-resolution mapping of a major QTL associated with resistance .
| | Author: Sujatha K Natarajkumar P Laha GS Mishra B Rao KS Viraktamath BC Kirti PB Hari Y Balachandran SM Rajendrakumar P Ram T Hajira SK Madhav MS Neeraja CN Sundaram RM | | Journal: Genet Res ( Camb ) Citation: V : 93 P : 397-408 Year: 2011 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22189605 Accession (PMID): 22189605 | | Abstract: SummaryThe cultivar Ajaya ( IET 8585 ) exhibits durable broad-spectrum resistance to bacterial blight ( BB ) disease of rice and is widely used as a resistance donor .
The present study was carried out to decipher the genetics of BB resistance in Ajaya and map the gene ( s ) conferring resistance .
Genetic analysis in the F2 indicated a quantitative/additive nature of resistance governed by two loci with equal effects .
Linked marker analysis and allelic tests revealed that one of the resistance genes is xa5 .
Sequence analysis of a 244 bp region of the second exon of the gene-encoding Transcription factor IIAgamma ( the candidate gene for xa5 ) confirmed the presence of xa5 .
Bulked-segregant analysis ( BSA ) revealed the putative location of the two quantitative trait loci ( QTLs ) /genes associated with resistance on chromosomes 5 and 8 .
Composite interval mapping located the first locus on Chr .
5S exactly in the genomic region spanned by xa5 and the second locus ( qtl BBR 8 . 1 ) on Chr .
8L .
Owing to its differential disease reaction with a set of seven hyper-virulent isolates of Xanthomonas oryzae , a map location on Chr .
8L , which was distinct from xa13 and data from allelism tests , the second resistance locus in Ajaya was determined to be novel and was designated as xaAj .
A contig map spanning xaAj was constructed in silico and the genomic region was delimited to a 13 . 5 kb physical interval In silico analysis of the genomic region spanning xaAj identified four putatively expressed candidate genes , one of which could be involved in imparting BB resistance in Ajaya along with xa5 .
| Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Sequence analysis of a 244 bp region of the second exon of the gene-encoding Transcription factor IIAgamma ( the candidate gene for xa5 ) confirmed the presence of xa5 .
[ Sen. 4, subscore: 1.00 ]: Linked marker analysis and allelic tests revealed that one of the resistance genes is xa5 .
[ Sen. 8, subscore: 1.00 ]: 5S exactly in the genomic region spanned by xa5 and the second locus ( qtl BBR 8 . 1 ) on Chr .
[ Sen. 12, subscore: 1.00 ]: A contig map spanning xaAj was constructed in silico and the genomic region was delimited to a 13 . 5 kb physical interval In silico analysis of the genomic region spanning xaAj identified four putatively expressed candidate genes , one of which could be involved in imparting BB resistance in Ajaya along with xa5 .
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Score: 4.00 |
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| Title: High resolution genetic mapping and candidate gene identification at the xa5 locus for bacterial blight resistance in rice ( Oryza sativa L ) .
| | Author: Blair MW Garris AJ Iyer AS Chapman B Kresovich S McCouch SR .
| | Journal: Theor . Appl . Genet . Citation: V : 107 ( 1 ) P : 62-73 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12677405 Accession (PMID): 12677405 | | Abstract: The xa5 resistance gene from rice provides recessive , race-specific resistance to bacterial blight of rice caused by the pathogen Xanthomonas oryzae pv oryzae .
A high-resolution genetic map of the chromosomal region surrounding xa5 was developed by placing 44 DNA markers on the distal end of rice chromosome 5 .
The basis for mapping was a PCR-based screening of 1 , 016 F ( 2 ) individuals derived from a cross between a near-isogenic line ( NIL ) and its corresponding recurrent parent to identify recombinants in the region .
Recombinant F ( 2 ) individuals were progeny tested using F ( 3 ) families inoculated with the Philippine strain PXO 61 of bacterial blight pathogen .
The xa5 gene was mapped to a 0 . 5-cM interval between the markers RS7 and RM611 , which spanned an interval of approximately 70 kb and contained a total of 11 open reading frames .
Sequence data for the locus was generated from an Indica ( the IR24 isoline , IRBB21 ) BAC covering part of the region and compared to other overlapping Indica ( cv 93-11 ) and Japonica ( cv Nipponbare ) sequences .
Candidate-gene analysis revealed that a basal transcription factor ( TFIIa ) , an ABC transporter , a tRNA synthase , a MAP kinase and a cysteine protease , as well as four unknown , hypothetical or putative proteins , are encoded at the locus and could be potential candidates for the resistance gene product .
The mechanism by which these genes could provide recessive , race-specific resistance will be elucidated by map-based cloning of the xa5 gene . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The xa5 resistance gene from rice provides recessive , race-specific resistance to bacterial blight of rice caused by the pathogen Xanthomonas oryzae pv oryzae .
[ Sen. 2, subscore: 1.00 ]: A high-resolution genetic map of the chromosomal region surrounding xa5 was developed by placing 44 DNA markers on the distal end of rice chromosome 5 .
[ Sen. 5, subscore: 1.00 ]: The xa5 gene was mapped to a 0 . 5-cM interval between the markers RS7 and RM611 , which spanned an interval of approximately 70 kb and contained a total of 11 open reading frames .
[ Sen. 8, subscore: 1.00 ]: The mechanism by which these genes could provide recessive , race-specific resistance will be elucidated by map-based cloning of the xa5 gene .
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Score: 4.00 |
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| Title: Identification of an avirulence gene , avrxa5 , from the rice pathogen Xanthomonas oryzae pv . oryzae .
| | Author: Zou H Zhao W Zhang X Han Y Zou L Chen G | | Journal: Sci China Life Sci Citation: V : 53 P : 1440-9 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21181346 Accession (PMID): 21181346 | | Abstract: Xanthomonas oryzae pv . oryzae , the causal agent of bacterial blight in rice , interacts with rice plants in a gene-for-gene manner .
The specificity of the interaction is dictated by avirulence ( avr ) genes in the pathogen and resistance ( R ) genes in the host To date , no avr genes that correspond to recessive R genes have been isolated .
We isolated an avrBs3/pthA family gene , avrxa5 , from our previously isolated clone p58 , which was originally from strain JXOIII .
The avrxa5 gene converted the PXO99 ( A ) strain from compatible to incompatible in rice cultivars containing the recessive xa5 gene , but not in those containing the dominant Xa5 gene .
Sequencing indicated that avrxa5 , which is highly similar to members of the avrBs3/pthA family , encodes a protein of 1238 amino acid residues with a conserved carboxy-terminal region containing three nuclear localization signals and a transcription activation domain .
It has 19 . 5 34-amino-acid direct repeats , but the 13th amino acid is missing in the fifth and ninth repetitive units .
Domain swapping of the repetitive regions between avrxa5 and avrXa7 changed the avirulence specificity of the genes in xa5 and Xa7 rice lines , respectively .
This indicates that avrxa5 is distinct from previously characterized avrBs3/pthA members .
The specificity of avrxa5 toward recessive xa5 in rice could help us better understand the molecular mechanisms of plant-pathogen specific interactions .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: The avrxa5 gene converted the PXO99 ( A ) strain from compatible to incompatible in rice cultivars containing the recessive xa5 gene , but not in those containing the dominant Xa5 gene .
[ Sen. 7, subscore: 1.00 ]: Domain swapping of the repetitive regions between avrxa5 and avrXa7 changed the avirulence specificity of the genes in xa5 and Xa7 rice lines , respectively .
[ Sen. 9, subscore: 1.00 ]: The specificity of avrxa5 toward recessive xa5 in rice could help us better understand the molecular mechanisms of plant-pathogen specific interactions .
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Score: 4.00 |
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| Title: Detection of bacterial blight resistance genes in basmati rice landraces .
| | Author: Ullah I Jamil S Iqbal MZ Shaheen HL Hasni SM Jabeen S Mehmood A Akhter M | | Journal: Genet Mol Res Citation: V : 11 P : 1960-6 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22869552 Accession (PMID): 22869552 | | Abstract: Aromatic basmati rice is vulnerable to bacterial blight disease .
Genes conferring resistance to bacterial blight have been identified in coarse rice ; however , their incorporation into basmati varieties compromises the prized basmati aroma .
We identified bacterial blight resistance genes Xa4 , xa5 , Xa7 , and xa13 in 52 basmati landraces and five basmati cultivars using PCR markers .
The Xa7 gene was found to be the most prevalent among the cultivars and landraces .
The cultivars Basmati-385 and Basmati-2000 also contained the Xa4 gene ; however , xa5 and xa13 were confined to landraces only .
Ten landraces were found to have multiple resistance genes .
Landraces Basmati-106 , Basmati-189 and Basmati-208 contained Xa4 and Xa7 genes .
Whereas , landraces Basmati-122 , Basmati-427 , Basmati-433 were observed to have xa5 and Xa7 genes .
Landraces Basmati-48 , Basmati-51A , Basmati-334 , and Basmati-370A possessed Xa7 and xa13 genes .
The use of landraces containing recessive genes xa5 and xa13 as donor parents in hybridization with cultivars Basmati-385 and Basmati-2000 , which contain the genes Xa4 and Xa7 , will expedite efforts to develop bacterial blight-resistant basmati rice cultivars through marker assisted selection , based on a pyramiding approach , without compromising aroma and grain quality .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: We identified bacterial blight resistance genes Xa4 , xa5 , Xa7 , and xa13 in 52 basmati landraces and five basmati cultivars using PCR markers .
[ Sen. 5, subscore: 1.00 ]: The cultivars Basmati-385 and Basmati-2000 also contained the Xa4 gene ; however , xa5 and xa13 were confined to landraces only .
[ Sen. 8, subscore: 1.00 ]: Whereas , landraces Basmati-122 , Basmati-427 , Basmati-433 were observed to have xa5 and Xa7 genes .
[ Sen. 10, subscore: 1.00 ]: The use of landraces containing recessive genes xa5 and xa13 as donor parents in hybridization with cultivars Basmati-385 and Basmati-2000 , which contain the genes Xa4 and Xa7 , will expedite efforts to develop bacterial blight-resistant basmati rice cultivars through marker assisted selection , based on a pyramiding approach , without compromising aroma and grain quality .
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Score: 3.00 |
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| Title: Marker assisted selection of bacterial blight resistance genes in rice .
| | Author: Davierwala AP Reddy AP Lagu MD Ranjekar PK Gupta VS .
| | Journal: Biochem . Genet . Citation: V : 39 ( 7-8 ) P : 261-78 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11590832 Accession (PMID): 11590832 | | Abstract: Bacterial leaf blight caused by Xanthomonas oryzae pv . oryzae is one of the most important diseases affecting rice production in Asia .
We were interested in surveying rice genotypes that are popularly used in the Indian breeding program for conferring resistance to bacterial blight , using 11 STMS and 6 STS markers .
The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes .
Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line .
Using the polymorphic markers obtained in the initial survey , marker-assisted selection was performed in the F3 population of a cross between IR-64 and IET-14444 to detect lines containing multiple resistance genes .
Of the 59 progeny lines analyzed , eight lines contained both the resistance genes , xa5 and Xa4 . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The basis of selection of these DNA markers was their close linkage to xa5 , xa13 , and Xa21 genes and their positions on the rice genetic map relative to bacterial blight resistance genes .
[ Sen. 4, subscore: 1.00 ]: Eight lines were found to contain the xa5 gene while two lines contained Xa21 gene and none of the lines contained the xa13 gene with the exception of its near-isogenic line .
[ Sen. 6, subscore: 1.00 ]: Of the 59 progeny lines analyzed , eight lines contained both the resistance genes , xa5 and Xa4 .
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Score: 3.00 |
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| Title: Are the dominant and recessive plant disease resistance genes similar?
A case study of rice R genes and Xanthomonas oryzae pv . oryzae races .
| | Author: Li ZK Sanchez A Angeles E Singh S Domingo J Huang N Khush GS .
| | Journal: Genetics Citation: V : 159 ( 2 ) P : 757-65 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11606550 Accession (PMID): 11606550 | | Abstract: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races .
Our results indicate that these two resistance components of rice plants were associated with the properties of the R genes .
The qualitative component of the R genes was reflected by their large effects against corresponding avirulent Xoo races .
The quantitative component of the R genes was their residual effects against corresponding virulent races and their epistatic effects , which together could lead to high-level resistance in a race-specific manner .
Our results revealed important differences between the different types of R genes .
Two R genes , Xa4 and Xa21 , showed complete dominance against the avirulent Xoo races and had large residual effects against virulent ones .
They acted independently and cumulatively , suggesting they are involved in different pathways of the rice defensive system .
The third R gene , xa5 , showed partial dominance or additivity to the avirulent Xoo races and had relatively small but significant residual effects against the virulent races .
In contrast , xa13 was completely recessive , had no residual effects against the virulent races , and showed more pronounced race specificity .
There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
Our results indicated that high-level and durable resistance to Xoo should be more efficiently achieved by pyramiding different types of R genes . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv . oryzae ( Xoo ) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance ( R ) genes ( Xa4 , xa5 , xa13 , and Xa21 ) and 12 Xoo races .
[ Sen. 8, subscore: 1.00 ]: The third R gene , xa5 , showed partial dominance or additivity to the avirulent Xoo races and had relatively small but significant residual effects against the virulent races .
[ Sen. 10, subscore: 1.00 ]: There was a strong interaction leading to increased resistance between xa13 and xa5 and between either of them and Xa4 or Xa21 , suggesting their regulatory roles in the rice defensive pathway ( s ) .
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