Score: 7.00 | Title: Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance .
| Author: Wang YS Pi LY Chen X Chakrabarty PK Jiang J De Leon AL Liu GZ Li L Benny U Oard J Ronald PC Song WY .
| Journal: Citation: V : ( ) P : Year: 2006 | Literature: oryza Field: abstract Doc ID: pub17172358 Accession (PMID): 17172358 | Abstract: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae .
We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase .
The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays .
XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively .
Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae .
Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein .
These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . | Matching Sentences: [ Sen. 3, subscore: 2.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae . We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase . The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . [ Sen. 2, subscore: 1.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae . We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase . The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . [ Sen. 4, subscore: 1.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae . We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase . The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . [ Sen. 5, subscore: 1.00 ]: XA21 is a receptor-like kinase protein in rice ( Oryza sativa ) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease , Xanthomonas oryzae pv oryzae . We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase . The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . [ Sen. 6, subscore: 1.00 ]: We identified XA21 binding protein 3 ( XB3 ) , an E3 ubiquitin ligase , as a substrate for the XA21 Ser and Thr kinase . The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance . [ Sen. 7, subscore: 1.00 ]: The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro , and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays . XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity , respectively . Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X oryzae pv oryzae . Furthermore , reduced levels of Xb3 lead to decreased levels of the XA21 protein . These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance .
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